Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection

Omana V. Nainan, Marina L. Khristova, Kwansoo Byun, Guoliang Xia, Patricia E. Taylor, Cladd E. Stevens, Harold S. Margolis

    Research output: Contribution to journalArticlepeer-review

    77 Citations (Scopus)

    Abstract

    Variants in the amino acid composition of the primary antibody-binding site of hepatitis B surface antigen (HBsAg) have been identified in a number of populations with chronic hepatitis B virus (HBV) infection. Direct sequencing of amplified or cloned PCR products, solid phase detection of sequence-specific PCR products (SP-PCR), and limiting dilution cloning PCR (LDC-PCR) were compared to determine their sensitivity in detecting differing concentrations of HBsAg variants. LDC-PCR had the greatest sensitivity and could detect HBsAg variants at a concentration of 0.1% of the total viral population. HBsAg variants were detected in 51% of infants with chronic HBV infection acquired after postexposure prophylaxis, and more than half of the variants were detected only by the most sensitive methods.

    Original languageEnglish
    Pages (from-to)319-327
    Number of pages9
    JournalJournal of Medical Virology
    Volume68
    Issue number3
    DOIs
    Publication statusPublished - 2002 Nov 1

    Keywords

    • "a" determinant mutants
    • Detection of HBsAg variants
    • HBsAg variants
    • Hepatitis B vaccine
    • Postexposure prophylaxis

    ASJC Scopus subject areas

    • Virology
    • Infectious Diseases

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