Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection

Omana V. Nainan, Marina L. Khristova, Kwan Soo Byun, Guoliang Xia, Patricia E. Taylor, Cladd E. Stevens, Harold S. Margolis

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Variants in the amino acid composition of the primary antibody-binding site of hepatitis B surface antigen (HBsAg) have been identified in a number of populations with chronic hepatitis B virus (HBV) infection. Direct sequencing of amplified or cloned PCR products, solid phase detection of sequence-specific PCR products (SP-PCR), and limiting dilution cloning PCR (LDC-PCR) were compared to determine their sensitivity in detecting differing concentrations of HBsAg variants. LDC-PCR had the greatest sensitivity and could detect HBsAg variants at a concentration of 0.1% of the total viral population. HBsAg variants were detected in 51% of infants with chronic HBV infection acquired after postexposure prophylaxis, and more than half of the variants were detected only by the most sensitive methods.

Original languageEnglish
Pages (from-to)319-327
Number of pages9
JournalJournal of Medical Virology
Volume68
Issue number3
DOIs
Publication statusPublished - 2002 Nov 1

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Keywords

  • "a" determinant mutants
  • Detection of HBsAg variants
  • HBsAg variants
  • Hepatitis B vaccine
  • Postexposure prophylaxis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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