Genipin inhibits the invasion and migration of colon cancer cells by the suppression of HIF-1α accumulation and VEGF expression

Suk young Lee, Hong Jun Kim, Sang Cheul Oh, Dae Hee Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Hypoxia-inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) play important roles in cancer progression in various cancer cell lines. Although genipin, a constituent of Gardenia fruit, has been shown to have anti-tumor activity, its role in the suppression of HIF-1 and its downstream target genes is not well understood. We examined the effect of genipin on the intracellular level of HIF-1α and extracellular level of VEGF using the colon cancer cell line HCT116. We observed that genipin suppressed the accumulation of HIF-1α under hypoxia in various cancer cell lines, including HCT116, via the modulation of protein degradation. Genipin also suppressed the expression of VEGF and the invasion of colon cancer cells by blocking the extracellular signal-regulated kinase signaling pathway. Taken together, our results provide new insights into the potential role of genipin in suppressing colon cancer progression.

Original languageEnglish
Pages (from-to)70-76
Number of pages7
JournalFood and Chemical Toxicology
Volume116
DOIs
Publication statusPublished - 2018 Jun 1

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Hypoxia-Inducible Factor 1
Cell Hypoxia
vascular endothelial growth factors
colorectal neoplasms
Colonic Neoplasms
Vascular Endothelial Growth Factor A
Cells
cell lines
Cell Line
Gardenia
Neoplasms
neoplasms
protein degradation
mitogen-activated protein kinase
Extracellular Signal-Regulated MAP Kinases
hypoxia
Fruits
Proteolysis
Tumors
Fruit

Keywords

  • Akt
  • ERK
  • Genipin
  • Hypoxia-inducible factor-1
  • Invasion
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Genipin inhibits the invasion and migration of colon cancer cells by the suppression of HIF-1α accumulation and VEGF expression. / Lee, Suk young; Kim, Hong Jun; Oh, Sang Cheul; Lee, Dae Hee.

In: Food and Chemical Toxicology, Vol. 116, 01.06.2018, p. 70-76.

Research output: Contribution to journalArticle

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AB - Hypoxia-inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) play important roles in cancer progression in various cancer cell lines. Although genipin, a constituent of Gardenia fruit, has been shown to have anti-tumor activity, its role in the suppression of HIF-1 and its downstream target genes is not well understood. We examined the effect of genipin on the intracellular level of HIF-1α and extracellular level of VEGF using the colon cancer cell line HCT116. We observed that genipin suppressed the accumulation of HIF-1α under hypoxia in various cancer cell lines, including HCT116, via the modulation of protein degradation. Genipin also suppressed the expression of VEGF and the invasion of colon cancer cells by blocking the extracellular signal-regulated kinase signaling pathway. Taken together, our results provide new insights into the potential role of genipin in suppressing colon cancer progression.

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