Genome sequence of type strain of Staphylococcus aureus subsp. aureus

Bong Soo Kim, Hana Yi, Jongsik Chun, Chang Jun Cha

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Staphylococcus aureus is a pathogen that causes food poisoning and community-associated infection with antibiotic resistance. This species is an indigenous intestinal microbe found in infants and not found in adult intestine. The relatively small genome size and rapid evolution of antibiotic resistance genes in the species have been drawing an increasing attention in public health. To extend our understanding of the species and use the genome data for comparative genomic studies, we sequenced the type strain of S. aureus subsp. aureus DSM 20231T. Results: Seventeen contigs were generated using hybrid assembly of sequences derived from the Roche 454 and Illumina systems. The length of the genome sequence was 2,902,619 bases with a G + C content of 32.8%. Among the 2,550 annotated CDSs, 44 CDSs were annotated to antibiotic resistance genes and 13 CDSs were related to methicillin resistance. It is interesting to note that this strain was first isolated in 1884 before methicillin was generally used on patients. Conclusions: The present study analyzed the genome sequence of S. aureus subsp. aureus type strain as the reference sequence for comparative genomic analyses of clinical isolates. Methicillin resistance genes found in the genome indicate the presence of antibiotic resistance mechanism prior to the usage of antibiotics. Further comparative genomic studies of methicillin-resistant strains based on this reference genome would help to understand the evolution of resistance mechanism and dissemination of resistance genes.

Original languageEnglish
Article number6
JournalGut Pathogens
Volume6
Issue number1
DOIs
Publication statusPublished - 2014 Mar 17

Fingerprint

Staphylococcus aureus
Microbial Drug Resistance
Methicillin Resistance
Genome
Genes
Genome Size
Methicillin
Foodborne Diseases
Base Composition
Intestines
Public Health
Anti-Bacterial Agents
Infection

Keywords

  • Genome sequencing
  • Hybrid assembly
  • Staphylococcus aureus subsp. aureus
  • Type strain

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases
  • Microbiology
  • Parasitology
  • Virology

Cite this

Genome sequence of type strain of Staphylococcus aureus subsp. aureus. / Kim, Bong Soo; Yi, Hana; Chun, Jongsik; Cha, Chang Jun.

In: Gut Pathogens, Vol. 6, No. 1, 6, 17.03.2014.

Research output: Contribution to journalArticle

Kim, Bong Soo ; Yi, Hana ; Chun, Jongsik ; Cha, Chang Jun. / Genome sequence of type strain of Staphylococcus aureus subsp. aureus. In: Gut Pathogens. 2014 ; Vol. 6, No. 1.
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