Genome structure and complete sequence of genomic RNA of Daphne virus S

B. Y. Lee, B. E. Min, J. H. Ha, M. Y. Lee, Kyung Hee Paek, K. H. Ryu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The complete genomic nucleotide sequence and structure of Daphne virus S (DVS), a daphne-infecting member of the genus Carlavirus, were determined. The genome of DVS was 8,739 nucleotides long, excluding the poly (A) tails. The genome of DVS contained six open reading frames coding for proteins of Mr 227∈kDa (viral replicase), 25∈kDa, 11∈kDa and 7∈kDa (triple gene block TGB) proteins 1, 2 and 3), 35∈kDa (coat protein; CP), and 12∈kDa from the 5′ to 3′ ends; respectively. This is the typical genome structure of members of the genus Carlavirus. Overall amino acid sequence similarities for the six ORFs of DVS were from 58.5% to 13.2% to those of the other carlaviruses. The 227∈kDa replicase of DVS shared 45.5-39.2% amino acid similarities to that of 8 other known carlaviruses. Results from phylogenetic analyses of viral replicases and CPs demonstrated that DVS is a close relative of Helenium virus S and Chrysanthemum virus B. A total of 13 isolates of DVS shared 100-95.9% identities for the amino acid level and 99.5-81.0% identities for the nucleotide level. This is the first report of the complete genome sequence and structure of DVS and supports the conclusion that DVS is a typical species of the genus Carlavirus.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalArchives of Virology
Volume151
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

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Daphne
RNA Viruses
Viruses
Carlavirus
Genes
Genome
RNA
Viral Structures
Nucleotides
Open Reading Frames
Amino Acids
Chrysanthemum
Cercopithecine Herpesvirus 1
Capsid Proteins
Amino Acid Sequence

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Genome structure and complete sequence of genomic RNA of Daphne virus S. / Lee, B. Y.; Min, B. E.; Ha, J. H.; Lee, M. Y.; Paek, Kyung Hee; Ryu, K. H.

In: Archives of Virology, Vol. 151, No. 1, 01.01.2006, p. 193-200.

Research output: Contribution to journalArticle

Lee, B. Y. ; Min, B. E. ; Ha, J. H. ; Lee, M. Y. ; Paek, Kyung Hee ; Ryu, K. H. / Genome structure and complete sequence of genomic RNA of Daphne virus S. In: Archives of Virology. 2006 ; Vol. 151, No. 1. pp. 193-200.
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