Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues

Xinmin Zhang, Duncan T. Odom, Seung-Hoi Koo, Michael D. Conkright, Gianluca Canettieri, Jennifer Best, Huaming Chen, Richard Jenner, Elizabeth Herbolsheimer, Elisabeth Jacobsen, Shilpa Kadam, Joseph R. Ecker, Beverly Emerson, John B. Hogenesch, Terry Unterman, Richard A. Young, Marc Montminy

Research output: Contribution to journalArticle

654 Citations (Scopus)

Abstract

Hormones and nutrients often induce genetic programs via signaling pathways that interface with gene-specific activators. Activation of the cAMP pathway, for example, stimulates cellular gene expression by means of the PKA-mediated phosphorylation of cAMP-response element binding protein (CREB) at Ser-133. Here, we use genome-wide approaches to characterize target genes that are regulated by CREB in different cellular contexts. CREB was found to occupy = 4,000 promoter sites in vivo, depending on the presence and methylation state of consensus cAMP response elements near the promoter. The profiles for CREB occupancy were very similar in different human tissues, and exposure to a cAMP agonist stimulated CREB phosphorylation over a majority of these sites. Only a small proportion of CREB target genes was induced by cAMP in any cell type, however, due in part to the preferential recruitment of the coactivator CREB-binding protein to those promoters. These results indicate that CREB phosphorylation alone is not a reliable predictor of target gene activation and that additional CREB regulatory partners are required for recruitment of the transcriptional apparatus to the promoter.

Original languageEnglish
Pages (from-to)4459-4464
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number12
DOIs
Publication statusPublished - 2005 Mar 22
Externally publishedYes

Fingerprint

Cyclic AMP Response Element-Binding Protein
Transcriptional Activation
Phosphorylation
Genome
Genes
Response Elements
Protein Binding
Methylation
Carrier Proteins
Hormones
Gene Expression
Food

Keywords

  • cAMP
  • cAMP-response element binding protein-binding protein
  • DNA methylation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues. / Zhang, Xinmin; Odom, Duncan T.; Koo, Seung-Hoi; Conkright, Michael D.; Canettieri, Gianluca; Best, Jennifer; Chen, Huaming; Jenner, Richard; Herbolsheimer, Elizabeth; Jacobsen, Elisabeth; Kadam, Shilpa; Ecker, Joseph R.; Emerson, Beverly; Hogenesch, John B.; Unterman, Terry; Young, Richard A.; Montminy, Marc.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 12, 22.03.2005, p. 4459-4464.

Research output: Contribution to journalArticle

Zhang, X, Odom, DT, Koo, S-H, Conkright, MD, Canettieri, G, Best, J, Chen, H, Jenner, R, Herbolsheimer, E, Jacobsen, E, Kadam, S, Ecker, JR, Emerson, B, Hogenesch, JB, Unterman, T, Young, RA & Montminy, M 2005, 'Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 12, pp. 4459-4464. https://doi.org/10.1073/pnas.0501076102
Zhang, Xinmin ; Odom, Duncan T. ; Koo, Seung-Hoi ; Conkright, Michael D. ; Canettieri, Gianluca ; Best, Jennifer ; Chen, Huaming ; Jenner, Richard ; Herbolsheimer, Elizabeth ; Jacobsen, Elisabeth ; Kadam, Shilpa ; Ecker, Joseph R. ; Emerson, Beverly ; Hogenesch, John B. ; Unterman, Terry ; Young, Richard A. ; Montminy, Marc. / Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 12. pp. 4459-4464.
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