Genome wide analysis of u-box e3 ubiquitin ligases in wheat (Triticum aestivum l.)

Dae Yeon Kim, Yong Jin Lee, Min Jeong Hong, Jae Ho Kim, Yong Weon Seo

Research output: Contribution to journalArticlepeer-review

Abstract

U-box E3 ligase genes play specific roles in protein degradation by post-translational modification in plant signaling pathways, developmental stages, and stress responses; however, little is known about U-box E3 genes in wheat. We identified 213 U-box E3 genes in wheat based on U-box and other functional domains in their genome sequences. The U-box E3 genes were distributed among 21 chromosomes and most showed high sequence homology with homoeologous U-box E3 genes. Synteny analysis of wheat U-box E3 genes was conducted with other plant species such as Brachypodium distachyon, barley, rice, Triricum uratu, and Aegilops tauschii. A total of 209 RNA-seq samples representing 22 tissue types, from grain, root, leaf, and spike samples across multiple time points, were analyzed for clustering of U-box E3 gene expression during developmental stages, and the genes responded differently in various tissues and developmental stages. In addition, expression analysis of U-box E3 genes under abiotic stress, including drought, heat, and both heat and drought, and cold conditions, was conducted to provide information on U-box E3 gene expression under specific stress conditions. This analysis of U-box E3 genes could provide valuable information to elucidate biological functions for a better understanding of U-box E3 genes in wheat.

Original languageEnglish
Article number2699
Pages (from-to)1-19
Number of pages19
JournalInternational journal of molecular sciences
Volume22
Issue number5
DOIs
Publication statusPublished - 2021 Mar 1

Keywords

  • Abiotic stress
  • Developmental stages
  • U-box E3 ligase
  • Wheat

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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