Genomic cloning and characterization of mitochondrial elongation factor Tu (EF-Tu) gene (tufM) from maize (Zea mays L.)

Kyu Ri Choi, Kwang Soo Roh, Jeong Kook Kim, Woong Soep Sim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have cloned and characterized a mitochondrial elongation factor Tu (EF-Tu) gene (tufM) in maize (Zea mays L.). This maize tufM gene encoded a polypeptide of 452 amino acid residues, consisting of a putative transit peptide of 55 residues and a mature EF-Tu of 397 residues. The coding region was composed of 12 exons and 11 introns that ranged from 76 to 1673 bp in length. The deduced amino acid sequence showed 85.9% and 61.2% identity with Arabidopsis mitochondrial EF-Tu and Arabidopsis chloroplast EF-Tu sequence respectively. The transcription initiation site was determined to be 165 bp upstream of the AUG initiation codon by primer extension analysis. Southern blot analysis revealed that the cloned EF-Tu gene was encoded by the members of small gene family in maize. Although this gene does not resemble the Arabidopsis nuclear tufA gene, which encodes the plastid EF-Tu, and does not contain sequence elements found in all cyanobacterial and plastid tufA genes, the predicted amino acid sequence includes an N-terminal extension that resembles a mitochondrial targeting sequence, and shares three unique sequence elements with mitochondrial EF-Tu's from Arabidopsis thaliana, Saccharomyces cerevisiae, and Homo sapiens. Therefore, we concluded that this gene encodes the maize mitochondrial EF-Tu. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)233-242
Number of pages10
JournalGene
Volume257
Issue number2
DOIs
Publication statusPublished - 2000 Oct 31

Fingerprint

Peptide Elongation Factor Tu
Zea mays
Organism Cloning
Arabidopsis
Genes
Plastids
Amino Acid Sequence
Peptides
Initiator Codon
Transcription Initiation Site
Chloroplasts
Southern Blotting
Introns
Saccharomyces cerevisiae
Exons
Amino Acids

Keywords

  • Genomic cloning
  • Intron
  • Primer extension
  • Promoter

ASJC Scopus subject areas

  • Genetics

Cite this

Genomic cloning and characterization of mitochondrial elongation factor Tu (EF-Tu) gene (tufM) from maize (Zea mays L.). / Choi, Kyu Ri; Roh, Kwang Soo; Kim, Jeong Kook; Sim, Woong Soep.

In: Gene, Vol. 257, No. 2, 31.10.2000, p. 233-242.

Research output: Contribution to journalArticle

Choi, Kyu Ri ; Roh, Kwang Soo ; Kim, Jeong Kook ; Sim, Woong Soep. / Genomic cloning and characterization of mitochondrial elongation factor Tu (EF-Tu) gene (tufM) from maize (Zea mays L.). In: Gene. 2000 ; Vol. 257, No. 2. pp. 233-242.
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