TY - JOUR
T1 - Genomic identification and molecular characterization of a non-mammalian TNFAIP8L2 gene from Oplegnathus fasciatus
AU - Umasuthan, Navaneethaiyer
AU - Revathy, Kasthuri Saranya
AU - Whang, Ilson
AU - Kim, Eunmi
AU - Oh, Myung Joo
AU - Jung, Sung Ju
AU - Lee, Jeong Ho
AU - Park, Hae Chul
AU - Lee, Jehee
N1 - Funding Information:
This research was supported by the National Fisheries Research and Development Institute ( RP-2013-BT-096 ) grant.
PY - 2014/5/25
Y1 - 2014/5/25
N2 - Tumor necrosis factor alpha-induced protein 8-like 2 (TNFAIP8L2) is a newly described negative immune regulator, whose enigmatic biological functions are not clearly understood. In the present study, the TNFAIP8L2 homolog of rock bream (Oplegnathus fasciatus) was identified and characterized. The genomic composition of rock bream TNFAIP8L2 (~. 6.7. kb) represented a tripartite arrangement in which three exons are interrupted by two introns. The rock bream TNFAIP8L2 transcript (1974. bp) possessed a coding sequence of 561. bp encoding a peptide of 186 amino acids. The predicted rock bream TNFAIP8L2 protein was 21.1. kDa and revealed the typical features of known TNFAIP8L2 members including the DED-like domain. Rock bream TNFAIP8L2 was composed of six α-helices and demonstrated a distinct folding pattern of the TNFAIP8L2 family. It showed a certain degree of homology and phylogenetic relationship with the corresponding tilapia counterpart. Based on an interspecies genomic organizational comparison of TNFAIP8L2 orthologs, they could be classified into two classes, with teleost and non-teleost origin respectively. While teleost TNFAIP8L2s manifest a tripartite arrangement, non-teleost counterparts demonstrate a dipartite structure suggesting the loss of an intron during the post-piscine speciation. Promoter proximal region of rock bream TNFAIP8L2 consisted of multiple immune responsive cis-regulatory elements. Analysis of basal transcription in eleven tissues revealed its constitutive expression in examined tissues with highest magnitude in the head kidney. The modulated temporal mRNA expression of rock bream TNFAIP8L2 in head kidney post-challenges with stimulants (LPS and poly I:C) and pathogens (Streptococcus iniae and irido virus) was stimulant-specific. Additionally, a drastic down-regulation of rock bream TNFAIP8L2 mRNA level occurred in blood cells collected from experimentally injured animals, and it was accompanied by a contemporaneous down-regulation of cytokines, TNF-α and TGFβ3. All these findings imply that rock bream TNFAIP8L2 is potentially responsible for immune and inflammatory modulation in rock bream.
AB - Tumor necrosis factor alpha-induced protein 8-like 2 (TNFAIP8L2) is a newly described negative immune regulator, whose enigmatic biological functions are not clearly understood. In the present study, the TNFAIP8L2 homolog of rock bream (Oplegnathus fasciatus) was identified and characterized. The genomic composition of rock bream TNFAIP8L2 (~. 6.7. kb) represented a tripartite arrangement in which three exons are interrupted by two introns. The rock bream TNFAIP8L2 transcript (1974. bp) possessed a coding sequence of 561. bp encoding a peptide of 186 amino acids. The predicted rock bream TNFAIP8L2 protein was 21.1. kDa and revealed the typical features of known TNFAIP8L2 members including the DED-like domain. Rock bream TNFAIP8L2 was composed of six α-helices and demonstrated a distinct folding pattern of the TNFAIP8L2 family. It showed a certain degree of homology and phylogenetic relationship with the corresponding tilapia counterpart. Based on an interspecies genomic organizational comparison of TNFAIP8L2 orthologs, they could be classified into two classes, with teleost and non-teleost origin respectively. While teleost TNFAIP8L2s manifest a tripartite arrangement, non-teleost counterparts demonstrate a dipartite structure suggesting the loss of an intron during the post-piscine speciation. Promoter proximal region of rock bream TNFAIP8L2 consisted of multiple immune responsive cis-regulatory elements. Analysis of basal transcription in eleven tissues revealed its constitutive expression in examined tissues with highest magnitude in the head kidney. The modulated temporal mRNA expression of rock bream TNFAIP8L2 in head kidney post-challenges with stimulants (LPS and poly I:C) and pathogens (Streptococcus iniae and irido virus) was stimulant-specific. Additionally, a drastic down-regulation of rock bream TNFAIP8L2 mRNA level occurred in blood cells collected from experimentally injured animals, and it was accompanied by a contemporaneous down-regulation of cytokines, TNF-α and TGFβ3. All these findings imply that rock bream TNFAIP8L2 is potentially responsible for immune and inflammatory modulation in rock bream.
KW - Genomic arrangement
KW - Immune/injury challenge
KW - Rock bream
KW - Transcriptional expression
KW - Tumor necrosis factor alpha-induced protein 8-like 2 (TNFAIP8L2)
UR - http://www.scopus.com/inward/record.url?scp=84897508485&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2014.02.047
DO - 10.1016/j.gene.2014.02.047
M3 - Article
C2 - 24583177
AN - SCOPUS:84897508485
SN - 0378-1119
VL - 542
SP - 52
EP - 63
JO - Gene
JF - Gene
IS - 1
ER -