Genomic identification and molecular characterization of a non-mammalian TNFAIP8L2 gene from Oplegnathus fasciatus

Navaneethaiyer Umasuthan, Kasthuri Saranya Revathy, Ilson Whang, Eunmi Kim, Myung Joo Oh, Sung Ju Jung, Jeong Ho Lee, Hae Chul Park, Jehee Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tumor necrosis factor alpha-induced protein 8-like 2 (TNFAIP8L2) is a newly described negative immune regulator, whose enigmatic biological functions are not clearly understood. In the present study, the TNFAIP8L2 homolog of rock bream (Oplegnathus fasciatus) was identified and characterized. The genomic composition of rock bream TNFAIP8L2 (~. 6.7. kb) represented a tripartite arrangement in which three exons are interrupted by two introns. The rock bream TNFAIP8L2 transcript (1974. bp) possessed a coding sequence of 561. bp encoding a peptide of 186 amino acids. The predicted rock bream TNFAIP8L2 protein was 21.1. kDa and revealed the typical features of known TNFAIP8L2 members including the DED-like domain. Rock bream TNFAIP8L2 was composed of six α-helices and demonstrated a distinct folding pattern of the TNFAIP8L2 family. It showed a certain degree of homology and phylogenetic relationship with the corresponding tilapia counterpart. Based on an interspecies genomic organizational comparison of TNFAIP8L2 orthologs, they could be classified into two classes, with teleost and non-teleost origin respectively. While teleost TNFAIP8L2s manifest a tripartite arrangement, non-teleost counterparts demonstrate a dipartite structure suggesting the loss of an intron during the post-piscine speciation. Promoter proximal region of rock bream TNFAIP8L2 consisted of multiple immune responsive cis-regulatory elements. Analysis of basal transcription in eleven tissues revealed its constitutive expression in examined tissues with highest magnitude in the head kidney. The modulated temporal mRNA expression of rock bream TNFAIP8L2 in head kidney post-challenges with stimulants (LPS and poly I:C) and pathogens (Streptococcus iniae and irido virus) was stimulant-specific. Additionally, a drastic down-regulation of rock bream TNFAIP8L2 mRNA level occurred in blood cells collected from experimentally injured animals, and it was accompanied by a contemporaneous down-regulation of cytokines, TNF-α and TGFβ3. All these findings imply that rock bream TNFAIP8L2 is potentially responsible for immune and inflammatory modulation in rock bream.

Original languageEnglish
Pages (from-to)52-63
Number of pages12
JournalGene
Volume542
Issue number1
DOIs
Publication statusPublished - 2014 May 25

Keywords

  • Genomic arrangement
  • Immune/injury challenge
  • Rock bream
  • Transcriptional expression
  • Tumor necrosis factor alpha-induced protein 8-like 2 (TNFAIP8L2)

ASJC Scopus subject areas

  • Genetics

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    Umasuthan, N., Revathy, K. S., Whang, I., Kim, E., Oh, M. J., Jung, S. J., Lee, J. H., Park, H. C., & Lee, J. (2014). Genomic identification and molecular characterization of a non-mammalian TNFAIP8L2 gene from Oplegnathus fasciatus. Gene, 542(1), 52-63. https://doi.org/10.1016/j.gene.2014.02.047