Abstract
Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50. 08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.
| Original language | English |
|---|---|
| Pages (from-to) | 2105-2108 |
| Number of pages | 4 |
| Journal | Archives of Virology |
| Volume | 156 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2011 Nov 1 |
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ASJC Scopus subject areas
- Virology
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Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2. / Lee, Young Duck; Park, Jong Hyun; Chang, Hyo-Ihl.
In: Archives of Virology, Vol. 156, No. 11, 01.11.2011, p. 2105-2108.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2
AU - Lee, Young Duck
AU - Park, Jong Hyun
AU - Chang, Hyo-Ihl
PY - 2011/11/1
Y1 - 2011/11/1
N2 - Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50. 08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.
AB - Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50. 08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.
UR - http://www.scopus.com/inward/record.url?scp=80155148706&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80155148706&partnerID=8YFLogxK
U2 - 10.1007/s00705-011-1096-7
DO - 10.1007/s00705-011-1096-7
M3 - Article
C2 - 21931999
AN - SCOPUS:80155148706
VL - 156
SP - 2105
EP - 2108
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 11
ER -