Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2

Young Duck Lee, Jong Hyun Park, Hyo-Ihl Chang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50. 08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.

Original languageEnglish
Pages (from-to)2105-2108
Number of pages4
JournalArchives of Virology
Volume156
Issue number11
DOIs
Publication statusPublished - 2011 Nov 1

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Cronobacter sakazakii
Bacteriophages
Sequence Analysis
Genes
Genome
Open Reading Frames
Myoviridae
Base Composition
Capsid Proteins
Enterobacteriaceae
Bioinformatics
Computational Biology
Polyacrylamide Gel Electrophoresis
Proteins
Electron microscopes
Swine
Electrons
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2. / Lee, Young Duck; Park, Jong Hyun; Chang, Hyo-Ihl.

In: Archives of Virology, Vol. 156, No. 11, 01.11.2011, p. 2105-2108.

Research output: Contribution to journalArticle

Lee, Young Duck ; Park, Jong Hyun ; Chang, Hyo-Ihl. / Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2. In: Archives of Virology. 2011 ; Vol. 156, No. 11. pp. 2105-2108.
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