Genomic structural characterization and transcriptional expression analysis of proteasome activator PA28α and PA28β subunits from Oplegnathus fasciatus

Saranya Revathy Kasthuri, Navaneethaiyer Umasuthan, Ilson Whang, Eunmi Kim, Hae Chul Park, Jehee Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Proteasomes are multicatalytic subunit complexes involved in the degradation of cytosolic proteins and antigen presentation. In this study, we have characterized the alpha and beta subunits of proteasome activator complex from rock bream at the molecular level. RbPA28α and RbPA28β possessed the characteristic features of the subunits identified from mammals and teleosts. The RbPA28α and RbPA28β proteasome subunits contained a proline-rich motif (Region A), subunit-specific insert in the region corresponding to the KEKE motif of the known PA28α (Region B), conserved activation loop (Region C), a potential protein kinase C recognition site (Region D) and a highly homologous C-terminal region (Region E) among all three PA28 subunits. Multiple sequence alignment and pairwise alignment revealed that RbPA28α and RbPA28β proteins shared high homology with the teleosts and mammals. RbPA28α and RbPA28β genome possessed 11 exons interrupted by 10 introns. In silico promoter analysis of RbPA28α and RbPA28β revealed various transcription factor-binding sites displaying their regulation under various stress conditions. Tissue distribution profiling showed a higher expression in blood and gills. Transcriptional expression analysis of RbPA28α and RbPA28β showed up-regulation in the immune tissues following LPS and poly I:C challenges, providing further evidence for the immunological role of RbPA28α and RbPA28β.

Original languageEnglish
Pages (from-to)1224-1234
Number of pages11
JournalFish and Shellfish Immunology
Volume35
Issue number4
DOIs
Publication statusPublished - 2013 Oct 1

Fingerprint

proteasome endopeptidase complex
Proteasome Endopeptidase Complex
genomics
Mammals
mammals
Tissue
polyinosinic-polycytidylic acid
Poly I-C
antigen presentation
protein kinase C
sequence alignment
tissue distribution
bream
protein degradation
Proline
Introns
Protein Kinase C
exons
proline
introns

Keywords

  • Antigen presentation in fish
  • Genome structure
  • Immune challenges
  • PA28α
  • PA28β

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

Genomic structural characterization and transcriptional expression analysis of proteasome activator PA28α and PA28β subunits from Oplegnathus fasciatus. / Kasthuri, Saranya Revathy; Umasuthan, Navaneethaiyer; Whang, Ilson; Kim, Eunmi; Park, Hae Chul; Lee, Jehee.

In: Fish and Shellfish Immunology, Vol. 35, No. 4, 01.10.2013, p. 1224-1234.

Research output: Contribution to journalArticle

Kasthuri, Saranya Revathy ; Umasuthan, Navaneethaiyer ; Whang, Ilson ; Kim, Eunmi ; Park, Hae Chul ; Lee, Jehee. / Genomic structural characterization and transcriptional expression analysis of proteasome activator PA28α and PA28β subunits from Oplegnathus fasciatus. In: Fish and Shellfish Immunology. 2013 ; Vol. 35, No. 4. pp. 1224-1234.
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