Genomic structure and analysis of promoter sequence of a mouse μ opioid receptor gene

Bon Hong Min, Lance B. Augustin, Roderick F. Felsheim, James A. Fuchs, Horace H. Loh

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

We have isolated mouse μ opioid receptor genomic clones (termed MOR) containing the entire amino acid coding sequence corresponding to rat MOR-1 cDNA, including additional 5' flanking sequence. The mouse MOR gene is >53 kb long, and the coding sequence is divided by three introns, with exon junctions in codons 95 and 213 and between codons 386 and 387. The first intron is >26 kb, the second is 0.8 kb, and the third is >12 kb. Multiple transcription initiation sites were observed, with four major sites confirmed by 5' rapid amplification of cDNA ends and RNase protection located between 291 and 268 bp upstream of the translation start codon. Comparison of the 5' flanking sequence with a transcription factor database revealed putative cis- acting regulatory elements for transcription factors affected by cAMP, as well as those involved in the action of gluco- and mineralocorticoids, cytokines, and immune-cell-specific factors.

Original languageEnglish
Pages (from-to)9081-9085
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number19
DOIs
Publication statusPublished - 1994 Sep 13
Externally publishedYes

Fingerprint

5' Flanking Region
Opioid Receptors
Codon
Introns
Sequence Analysis
Transcription Factors
Complementary DNA
Mineralocorticoids
Initiator Codon
Transcription Initiation Site
Ribonucleases
Glucocorticoids
Genes
Amino Acid Sequence
Exons
Clone Cells
Databases
Cytokines

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Genomic structure and analysis of promoter sequence of a mouse μ opioid receptor gene. / Min, Bon Hong; Augustin, Lance B.; Felsheim, Roderick F.; Fuchs, James A.; Loh, Horace H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 19, 13.09.1994, p. 9081-9085.

Research output: Contribution to journalArticle

Min, Bon Hong ; Augustin, Lance B. ; Felsheim, Roderick F. ; Fuchs, James A. ; Loh, Horace H. / Genomic structure and analysis of promoter sequence of a mouse μ opioid receptor gene. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 19. pp. 9081-9085.
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