Genomic structure and analysis of promoter sequence of a mouse μ opioid receptor gene

Bon H. Min, Lance B. Augustin, Roderick F. Felsheim, James A. Fuchs, Horace H. Loh

    Research output: Contribution to journalArticlepeer-review

    160 Citations (Scopus)

    Abstract

    We have isolated mouse μ opioid receptor genomic clones (termed MOR) containing the entire amino acid coding sequence corresponding to rat MOR-1 cDNA, including additional 5' flanking sequence. The mouse MOR gene is >53 kb long, and the coding sequence is divided by three introns, with exon junctions in codons 95 and 213 and between codons 386 and 387. The first intron is >26 kb, the second is 0.8 kb, and the third is >12 kb. Multiple transcription initiation sites were observed, with four major sites confirmed by 5' rapid amplification of cDNA ends and RNase protection located between 291 and 268 bp upstream of the translation start codon. Comparison of the 5' flanking sequence with a transcription factor database revealed putative cis- acting regulatory elements for transcription factors affected by cAMP, as well as those involved in the action of gluco- and mineralocorticoids, cytokines, and immune-cell-specific factors.

    Original languageEnglish
    Pages (from-to)9081-9085
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume91
    Issue number19
    DOIs
    Publication statusPublished - 1994 Sep 13

    ASJC Scopus subject areas

    • General

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