Genomic structure and transcriptional studies on the mouse ribosomal protein S3 gene: Expression of U15 small nucleolar RNA

Seong Min Lee, Mijin Kim, Eun Pyo Moon, Byeong Jae Lee, Jin Young Choi, Joon Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ribosomal protein S3 (rpS3) is a multifunctional ribosomal protein (RP) which is known to function as a DNA repair endonuclease as well as an RP. Recently, it was reported that rpS3 is involved in apoptosis. We identified the complete 4760 base pair genomic structure of the mouse rpS3 gene, which is composed of 7 exons and 6 introns. Promoter study revealed that transcription of the mouse rpS3 gene started at two C residues embedded in the 5′-terminal oligopyrimidine tract (5′-TOP); this was then compared with the human counterpart. Functional U15 small nucleolar RNAs (snoRNAs) were expressed from the first and the fifth introns. About 300 base pairs (bps) upstream of the 5′-untranslated region (5′-UTR) of the mouse rpS3 gene was sufficient to show maximum transcription activity. This report shows the conservation of the genomic structure of the rpS3 gene in vertebrates and characteristics of its promoter similar to those of promoters of other mammalian RPs.

Original languageEnglish
Pages (from-to)12-20
Number of pages9
JournalGene
Volume368
Issue number1-2
DOIs
Publication statusPublished - 2006 Mar 1

Fingerprint

Small Nucleolar RNA
Gene Expression
Ribosomal Proteins
Base Pairing
Introns
Genes
5' Untranslated Regions
Deoxyribonuclease I
DNA Repair
Vertebrates
ribosomal protein S3
Exons
Apoptosis

Keywords

  • Genomic organization
  • Ribosomal protein S3 promoter
  • Transcription start site
  • U15 snoRNA

ASJC Scopus subject areas

  • Genetics

Cite this

Genomic structure and transcriptional studies on the mouse ribosomal protein S3 gene : Expression of U15 small nucleolar RNA. / Lee, Seong Min; Kim, Mijin; Moon, Eun Pyo; Lee, Byeong Jae; Choi, Jin Young; Kim, Joon.

In: Gene, Vol. 368, No. 1-2, 01.03.2006, p. 12-20.

Research output: Contribution to journalArticle

Lee, Seong Min ; Kim, Mijin ; Moon, Eun Pyo ; Lee, Byeong Jae ; Choi, Jin Young ; Kim, Joon. / Genomic structure and transcriptional studies on the mouse ribosomal protein S3 gene : Expression of U15 small nucleolar RNA. In: Gene. 2006 ; Vol. 368, No. 1-2. pp. 12-20.
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