Genomic structure of the mouse δ opioid receptor gene

Lance B. Augustin, Roderick F. Felsheim, Bon Hong Min, Stephanie M. Fuchs, James A. Fuchs, Horace H. Loh

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Using mouse δ opioid receptor (DOR) cDNA sequence to probe genomic libraries in bacteriophage λ and P1 vectors, clones traversing the entire DOR coding sequence and 5' and 3' flanking regions were isolated. Genomic sequence encoding mature DOR message, including 5' and 3' untranslated sequence, is divided by two introns of 26 kb and 3 kb, resulting in the gene occupying 32 kb of chromosomal DNA. Multiple putative transcription initiation sites were located, by RNase protection assay, in TATA-less G+C rich sequence between 390 and 140 nucleotides upstream from the ATG translation start codon. A polyadenylation site was located 1.24 kb downstream from the TGA translation stop codon. Examination of 1.3 kb of 5' flanking sequence revealed potential binding sites for several known transcription factors including: Sp1, Ap-2, NF-κB, NF-IL6, and NGFI-B.

Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume207
Issue number1
DOIs
Publication statusPublished - 1995 Mar 2
Externally publishedYes

Fingerprint

5' Flanking Region
Opioid Receptors
Genes
Bacteriophage P1
CCAAT-Enhancer-Binding Protein-beta
Sp1 Transcription Factor
3' Flanking Region
Polyadenylation
Bacteriophages
Initiator Codon
Genomic Library
Terminator Codon
Transcription Initiation Site
Ribonucleases
Introns
Assays
Transcription Factors
Nucleotides
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Augustin, L. B., Felsheim, R. F., Min, B. H., Fuchs, S. M., Fuchs, J. A., & Loh, H. H. (1995). Genomic structure of the mouse δ opioid receptor gene. Biochemical and Biophysical Research Communications, 207(1), 111-119. https://doi.org/10.1006/bbrc.1995.1160

Genomic structure of the mouse δ opioid receptor gene. / Augustin, Lance B.; Felsheim, Roderick F.; Min, Bon Hong; Fuchs, Stephanie M.; Fuchs, James A.; Loh, Horace H.

In: Biochemical and Biophysical Research Communications, Vol. 207, No. 1, 02.03.1995, p. 111-119.

Research output: Contribution to journalArticle

Augustin, Lance B. ; Felsheim, Roderick F. ; Min, Bon Hong ; Fuchs, Stephanie M. ; Fuchs, James A. ; Loh, Horace H. / Genomic structure of the mouse δ opioid receptor gene. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 207, No. 1. pp. 111-119.
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