Gentisyl alcohol inhibits proliferation and induces apoptosis via mitochondrial dysfunction and regulation of MAPK and PI3K/AKT pathways in epithelial ovarian cancer cells

Jiyeon Ham, Whasun Lim, Kyeongwon Kim, Young Mok Heo, Seung Mok Ryu, Dongho Lee, Jae-Jin Kim, Gwonhwa Song

Research output: Contribution to journalArticle

Abstract

Ovarian cancer is one of the prevalent gynecological cancers occurring in women. In particular, the efficiency of standard therapeutic methods decreases when recurrence and chemoresistance ensue. To assist standard anti-cancer agents in the cure of ovarian cancer, development and application of new compounds such as small molecules or natural products are required. Gentisyl alcohol is one of the secondary metabolites that can be obtained by purification from bacteria or fungi and is known to have antibacterial, antifungal, antiviral, and anti-cancer effects. In the present study, we verified the effect of gentisyl alcohol derived from marine Arthrinium sp. on suppressing proliferation and inducing apoptosis via DNA fragmentation in human ovarian cancers cells (ES2 and OV90 cells). We also confirmed that there was an accumulation of sub-G1 cells and a loss of mitochondrial membrane potential with calcium dysregulation in gentisyl alcohol-treated ovarian cancer cells. Moreover, gentisyl alcohol up-regulated signal transduction of MAPK and PI3K/AKT pathways. Collectively, our results demonstrated the possibility of gentisyl alcohol as a novel therapeutic agent for human ovarian cancer.

Original languageEnglish
Article number331
JournalMarine Drugs
Volume17
Issue number6
DOIs
Publication statusPublished - 2019 Jun 3

Fingerprint

Phosphatidylinositol 3-Kinases
Ovarian Neoplasms
Apoptosis
Neoplasms
Mitochondrial Membrane Potential
DNA Fragmentation
Biological Products
Antiviral Agents
Signal Transduction
Fungi
2,5-dihydroxybenzyl alcohol
Ovarian epithelial cancer
Calcium
Bacteria
Recurrence
Therapeutics

Keywords

  • Calcium dysregulation
  • Gentisyl alcohol
  • Mitochondrial dysfunction
  • Ovarian cancer

ASJC Scopus subject areas

  • Drug Discovery

Cite this

Gentisyl alcohol inhibits proliferation and induces apoptosis via mitochondrial dysfunction and regulation of MAPK and PI3K/AKT pathways in epithelial ovarian cancer cells. / Ham, Jiyeon; Lim, Whasun; Kim, Kyeongwon; Heo, Young Mok; Ryu, Seung Mok; Lee, Dongho; Kim, Jae-Jin; Song, Gwonhwa.

In: Marine Drugs, Vol. 17, No. 6, 331, 03.06.2019.

Research output: Contribution to journalArticle

@article{28cb656edec14918a762003a44b1e87d,
title = "Gentisyl alcohol inhibits proliferation and induces apoptosis via mitochondrial dysfunction and regulation of MAPK and PI3K/AKT pathways in epithelial ovarian cancer cells",
abstract = "Ovarian cancer is one of the prevalent gynecological cancers occurring in women. In particular, the efficiency of standard therapeutic methods decreases when recurrence and chemoresistance ensue. To assist standard anti-cancer agents in the cure of ovarian cancer, development and application of new compounds such as small molecules or natural products are required. Gentisyl alcohol is one of the secondary metabolites that can be obtained by purification from bacteria or fungi and is known to have antibacterial, antifungal, antiviral, and anti-cancer effects. In the present study, we verified the effect of gentisyl alcohol derived from marine Arthrinium sp. on suppressing proliferation and inducing apoptosis via DNA fragmentation in human ovarian cancers cells (ES2 and OV90 cells). We also confirmed that there was an accumulation of sub-G1 cells and a loss of mitochondrial membrane potential with calcium dysregulation in gentisyl alcohol-treated ovarian cancer cells. Moreover, gentisyl alcohol up-regulated signal transduction of MAPK and PI3K/AKT pathways. Collectively, our results demonstrated the possibility of gentisyl alcohol as a novel therapeutic agent for human ovarian cancer.",
keywords = "Calcium dysregulation, Gentisyl alcohol, Mitochondrial dysfunction, Ovarian cancer",
author = "Jiyeon Ham and Whasun Lim and Kyeongwon Kim and Heo, {Young Mok} and Ryu, {Seung Mok} and Dongho Lee and Jae-Jin Kim and Gwonhwa Song",
year = "2019",
month = "6",
day = "3",
doi = "10.3390/md17060331",
language = "English",
volume = "17",
journal = "Marine Drugs",
issn = "1660-3397",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "6",

}

TY - JOUR

T1 - Gentisyl alcohol inhibits proliferation and induces apoptosis via mitochondrial dysfunction and regulation of MAPK and PI3K/AKT pathways in epithelial ovarian cancer cells

AU - Ham, Jiyeon

AU - Lim, Whasun

AU - Kim, Kyeongwon

AU - Heo, Young Mok

AU - Ryu, Seung Mok

AU - Lee, Dongho

AU - Kim, Jae-Jin

AU - Song, Gwonhwa

PY - 2019/6/3

Y1 - 2019/6/3

N2 - Ovarian cancer is one of the prevalent gynecological cancers occurring in women. In particular, the efficiency of standard therapeutic methods decreases when recurrence and chemoresistance ensue. To assist standard anti-cancer agents in the cure of ovarian cancer, development and application of new compounds such as small molecules or natural products are required. Gentisyl alcohol is one of the secondary metabolites that can be obtained by purification from bacteria or fungi and is known to have antibacterial, antifungal, antiviral, and anti-cancer effects. In the present study, we verified the effect of gentisyl alcohol derived from marine Arthrinium sp. on suppressing proliferation and inducing apoptosis via DNA fragmentation in human ovarian cancers cells (ES2 and OV90 cells). We also confirmed that there was an accumulation of sub-G1 cells and a loss of mitochondrial membrane potential with calcium dysregulation in gentisyl alcohol-treated ovarian cancer cells. Moreover, gentisyl alcohol up-regulated signal transduction of MAPK and PI3K/AKT pathways. Collectively, our results demonstrated the possibility of gentisyl alcohol as a novel therapeutic agent for human ovarian cancer.

AB - Ovarian cancer is one of the prevalent gynecological cancers occurring in women. In particular, the efficiency of standard therapeutic methods decreases when recurrence and chemoresistance ensue. To assist standard anti-cancer agents in the cure of ovarian cancer, development and application of new compounds such as small molecules or natural products are required. Gentisyl alcohol is one of the secondary metabolites that can be obtained by purification from bacteria or fungi and is known to have antibacterial, antifungal, antiviral, and anti-cancer effects. In the present study, we verified the effect of gentisyl alcohol derived from marine Arthrinium sp. on suppressing proliferation and inducing apoptosis via DNA fragmentation in human ovarian cancers cells (ES2 and OV90 cells). We also confirmed that there was an accumulation of sub-G1 cells and a loss of mitochondrial membrane potential with calcium dysregulation in gentisyl alcohol-treated ovarian cancer cells. Moreover, gentisyl alcohol up-regulated signal transduction of MAPK and PI3K/AKT pathways. Collectively, our results demonstrated the possibility of gentisyl alcohol as a novel therapeutic agent for human ovarian cancer.

KW - Calcium dysregulation

KW - Gentisyl alcohol

KW - Mitochondrial dysfunction

KW - Ovarian cancer

UR - http://www.scopus.com/inward/record.url?scp=85067453945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067453945&partnerID=8YFLogxK

U2 - 10.3390/md17060331

DO - 10.3390/md17060331

M3 - Article

C2 - 31163640

AN - SCOPUS:85067453945

VL - 17

JO - Marine Drugs

JF - Marine Drugs

SN - 1660-3397

IS - 6

M1 - 331

ER -