Glabretal-type triterpenoid from the root bark of Dictamnus dasycarpus ameliorates collagen-induced arthritis by inhibiting Erk-dependent lymphocyte proliferation

Sang Pil Choi, Chang Yong Choi, Kyungmin Park, Nahyun Kim, Hyun-Seuk Moon, Dongho Lee, Taehoon Chun

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4 Citations (Scopus)

Abstract

Ethnopharmacological relevance The root bark of Dictamnus dasycarpus Turcz. (Rutaceae) has been used as a traditional herbal medicine to treat various inflammatory diseases in East Asia. We have showed previously that a glabretal type triterpenoid (dictabretol A) from D. dasycarpus root bark has immunosuppressive activity. Aim of the study This study was conducted to define the molecular mechanism of how dictabretol A inhibits lymphocyte proliferation and to evaluate the therapeutic efficacy of dictabretol A in an animal model of rheumatoid arthritis. Materials and methods Various murine immune cells (T cells, B cells, and macrophages) and splenocytes were used to study the anti-proliferative effect of dictabretol A in vitro. A collagen-induced arthritis model was also used to examine the therapeutic effect of dictabretol A in vivo. Results Dictabretol A specifically inhibited lymphocyte proliferation by blocking the cell cycle transition from the G1 to the S phase. This effect was achieved by blocking Erk1/2, nuclear factor kappa B, and the C-myc axis of cell cycle progression. Further dictabretol A treatment alleviated the severity of collagen-induced arthritis. Conclusion Our results reveal the molecular mechanism for the anti-lymphoproliferative effect of dictabretol A and show the therapeutic efficacy of dictabretol A for rheumatoid arthritis.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalJournal of Ethnopharmacology
Volume178
DOIs
Publication statusPublished - 2016 Feb 3

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Keywords

  • Cell cycle
  • Collagen inducedarthritis
  • DictabretolA
  • Lymphocyte
  • Signal transductio

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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