Gliadin-specific IgE in wheat-dependent exercise-induced anaphylaxis

Jae Young Lee, Sungho Yoon, Young Min Ye, Young Hur Gyu, Seunghyun Kim, Hae Sim Park

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Food-dependent exercise-induced anaphylaxis is a unique form of food allergy. Recent studies indicate that gliadin is a major allergen in patients with wheat-dependent exercise-induced anaphylaxis (WDEIA). We evaluated the value of serum gliadin-specific IgE in the diagnosis of WDEIA, as well as the characteristics and digestibilities of the IgE-binding components of gliadin. We analyzed 32 adult subjects sensitized to wheat; 26 were asymptomatic sensitizers and 6 had WDEIA. Wheat flour and gliadin-specific IgE levels were measured, and corresponding receiver operator characteristics (ROC) curves were determined. Patients with WDEIA had higher levels of gliadin-specific IgE than patients without WDEIA (p = 0.003). The area under the ROC curve for gliadin-specific IgE was 0.872, which suggested this assay could be used as a supplementary test for the diagnosis of WDEIA. IgE immunoblot analyses of reactions to wheat and gliadin extracts were compared both with and without simulated gastric fluid (SGF) and intestinal fluid incubation (SIF). All six WDEIA patients had high IgE binding signals to 50 kDa of gliadin extract on IgE immunoblots. This binding was suppressed by SGF, although minimal inhibition was noted with SIF incubation.

    Original languageEnglish
    Pages (from-to)614-621
    Number of pages8
    JournalAllergy and Asthma Proceedings
    Volume29
    Issue number6
    DOIs
    Publication statusPublished - 2008 Nov

    Keywords

    • Anaphylaxis
    • Digestive enzyme
    • Exercise
    • Food allergy
    • Food-dependent exercise-induced anaphylaxis
    • Gliadin
    • IgE
    • ROC curve
    • Wheat

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Pulmonary and Respiratory Medicine

    Fingerprint

    Dive into the research topics of 'Gliadin-specific IgE in wheat-dependent exercise-induced anaphylaxis'. Together they form a unique fingerprint.

    Cite this