Glioma stem cells and their non-stem differentiated glioma cells exhibit differences in mitochondrial structure and function

Eun Jung Kim, Xiong Jin, Ock Ran Kim, Seok Won Ham, Sung Hye Park, Hyunggee Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Although mitochondria play an important role in cell survival, their biological significance in differentiated and undifferentiated cells is not well known. In the present study, we compared the differences in the structure and function of mitochondria between undifferentiated cancer stem cells and differentiated cancer cells. Glioma stem cells (GSCs), when grown under serum culture conditions, demonstrated a decrease in stem cell marker expression and tumor sphere forming ability, while showing an increase in differentiated cell markers. Transmission electron microscopy analysis revealed that the number of mitochondria with distinct cristae and electron-dense matrices increased significantly in the non-stem differentiated glioma cells when compared to their undifferentiated GSCs. Bioinformatic analysis revealed that the glucose metabolic process gene signature was enriched in gene pools that had an increased number of stem cells. Additionally, qRT-PCR analysis revealed that the expression of various glucose metabolism genes was higher in GSCs than in non-stem differentiated glioma cells. Altogether, our results suggest that GSCs have immature mitochondria when compared to differentiated glioma cells. Notably, GSCs prefer a relatively higher glucose metabolism, which implies that they utilize different mitochondrial biosynthesis and metabolic pathways when compared to differentiated glioma cells.

Original languageEnglish
Pages (from-to)411-416
Number of pages6
JournalOncology Reports
Volume39
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Glioma
Stem Cells
Mitochondria
Glucose
Gene Pool
Neoplastic Stem Cells
Tumor Biomarkers
Metabolic Networks and Pathways
Computational Biology
Transmission Electron Microscopy
Genes
Cell Survival
Electrons
Polymerase Chain Reaction
Serum

Keywords

  • Glioblastoma
  • Glioma stem cells
  • Glucose metabolism
  • Mitochondrial structure
  • Non-stem differentiated glioma cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Glioma stem cells and their non-stem differentiated glioma cells exhibit differences in mitochondrial structure and function. / Kim, Eun Jung; Jin, Xiong; Kim, Ock Ran; Ham, Seok Won; Park, Sung Hye; Kim, Hyunggee.

In: Oncology Reports, Vol. 39, No. 1, 01.01.2018, p. 411-416.

Research output: Contribution to journalArticle

Kim, Eun Jung ; Jin, Xiong ; Kim, Ock Ran ; Ham, Seok Won ; Park, Sung Hye ; Kim, Hyunggee. / Glioma stem cells and their non-stem differentiated glioma cells exhibit differences in mitochondrial structure and function. In: Oncology Reports. 2018 ; Vol. 39, No. 1. pp. 411-416.
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