Global gene response in Saccharomyces cerevisiae exposed to silver nanoparticles

Javed H. Niazi, Byoung In Sang, Yeon Seok Kim, Man Bock Gu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Silver nanoparticles (AgNPs), exhibiting a broad size range and morphologies with highly reactive facets, which are widely applicable in real-life but not fully verified for biosafety and ecotoxicity, were subjected to report transcriptome profile in yeast Saccharomyces cerevisiae. A large number of genes accounted for ∼3% and ∼5% of the genome affected by AgNPs and Ag-ions, respectively. Principal component and cluster analysis suggest that the different physical forms of Ag were the major cause in differential expression profile. Among 90 genes affected by both AgNPs and Ag-ions, metalloprotein mediating high resistance to copper (CUP1-1 and CUP1-2) were strongly induced by AgNPs (∼45-folds) and Ag-ions (∼22-folds), respectively. A total of 17 genes, responsive to chemical stimuli, stress, and transport processes, were differentially induced by AgNPs. The differential expression was also seen with Ag-ions that affected 73 up- and 161 downregulating genes, and most of these were involved in ion transport and homeostasis. This study provides new information on the knowledge for impact of nanoparticles on living microorganisms that can be extended to other nanoparticles.

Original languageEnglish
Pages (from-to)1278-1291
Number of pages14
JournalApplied Biochemistry and Biotechnology
Volume164
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1

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Keywords

  • Microarray
  • Nanotoxicity
  • Silver nanoparticles
  • Transcriptome analysis
  • Yeast genome analysis

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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