Global Mapping of Protein–Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells

Danyang Wang, Shubo Du, Amaury Cazenave-Gassiot, Jingyan Ge, Jun Seok Lee, Markus R. Wenk, Shao Q. Yao

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein–lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein–phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.

Original languageEnglish
Pages (from-to)5829-5833
Number of pages5
JournalAngewandte Chemie - International Edition
Volume56
Issue number21
DOIs
Publication statusPublished - 2017 May 15
Externally publishedYes

Keywords

  • click chemistry
  • phosphatidylcholine
  • photo-crosslinking
  • protein–lipid interactions
  • quantitative proteomics

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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