Global metabolic profiling of plant cell wall polysaccharide degradation by Saccharophagus degradans

Min Hye Shin, Do Yup Lee, Kirsten Skogerson, Gert Wohlgemuth, In-Geol Choi, Oliver Fiehn, Kyoung Heon Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Plant cell wall polysaccharides can be used as the main feedstock for the production of biofuels. Saccharophagus degradans 2-40 is considered to be a potent system for the production of sugars from plant biomass due to its high capability to degrade many complex polysaccharides. To understand the degradation metabolism of plant cell wall polysaccharides by S. degradans, the cell growth, enzyme activity profiles, and the metabolite profiles were analyzed by gas chromatography-time of flight mass spectrometry using different carbon sources including cellulose, xylan, glucose, and xylose. The specific activity of cellulase was only found to be significantly higher when cellulose was used as the sole carbon source, but the xylanase activity increased when xylan, xylose, or cellulose was used as the carbon source. In addition, principal component analysis of 98 identified metabolites in S. degradans revealed four distinct groups that differed based on the carbon source used. Furthermore, metabolite profiling showed that the use of cellulose or xylan as polysaccharides led to increased abundances of fatty acids, nucleotides and glucuronic acid compared to the use of glucose or xylose. Finally, intermediates in the pentose phosphate pathway seemed to be up-regulated on xylose or xylan when compared to those on glucose or cellulose. Such metabolic responses of S. degradans under plant cell wall polysaccharides imply that its metabolic system is transformed to more efficiently degrade polysaccharides and conserve energy. This study demonstrates that the gas chromatography-time of flight mass spectrometrybased global metabolomics are useful for understanding microbial metabolism and evaluating its fermentation characteristics.

Original languageEnglish
Pages (from-to)477-488
Number of pages12
JournalBiotechnology and Bioengineering
Volume105
Issue number3
DOIs
Publication statusPublished - 2010 Feb 15

Fingerprint

Plant Cells
Polysaccharides
Cell Wall
Xylans
Cellulose
Xylose
Degradation
Carbon
Metabolites
Glucose
Metabolism
Gas chromatography
Gas Chromatography
Pentoses
Pentose Phosphate Pathway
Glucuronic Acid
Metabolomics
Biofuels
Cellulase
Enzyme activity

Keywords

  • Cellulose
  • GC-TOF MS
  • Metabolomics
  • Saccharophgus degradans
  • Xylan

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Global metabolic profiling of plant cell wall polysaccharide degradation by Saccharophagus degradans. / Shin, Min Hye; Lee, Do Yup; Skogerson, Kirsten; Wohlgemuth, Gert; Choi, In-Geol; Fiehn, Oliver; Kim, Kyoung Heon.

In: Biotechnology and Bioengineering, Vol. 105, No. 3, 15.02.2010, p. 477-488.

Research output: Contribution to journalArticle

Shin, Min Hye ; Lee, Do Yup ; Skogerson, Kirsten ; Wohlgemuth, Gert ; Choi, In-Geol ; Fiehn, Oliver ; Kim, Kyoung Heon. / Global metabolic profiling of plant cell wall polysaccharide degradation by Saccharophagus degradans. In: Biotechnology and Bioengineering. 2010 ; Vol. 105, No. 3. pp. 477-488.
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