Global metabolite profiling of agarose degradation by Saccharophagus degradans 2-40

Min Hye Shin, Do Yup Lee, Gert Wohlgemuth, In-Geol Choi, Oliver Fiehn, Kyoung Heon Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Saccharophagus degradans is a potent degrader of marine and plant cell wall polysaccharides. In particular, it is capable of degrading and metabolizing agarose that is the main component of marine red algae. To understand its degradation and metabolism of agarose along with the agarase expression profile, S. degradans was grown using different carbon sources including galactose, agarose, glucose and cellulose. The metabolite profiling was conducted by using GC-TOF MS and in-house programmed database, BinBase. When the metabolite profiles of cells on galactose and agarose were compared, principal component analysis of 133 identified metabolites revealed clear separations between the groups on galactose and agarose. S. degradans grown on agarose was found to use different carbon catabolic pathways from that grown on other carbon sources. The metabolite profile of cells grown using galactose had increased abundances of glycerol, glycerol derivatives and fatty acids. The use of polysaccharides such as agarose or cellulose led to the increased abundances of amino acids and intermediates of nucleotide biosynthesis.

Original languageEnglish
Pages (from-to)156-168
Number of pages13
JournalNew Biotechnology
Volume27
Issue number2
DOIs
Publication statusPublished - 2010 May 31

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Metabolites
Sepharose
Degradation
Galactose
Polysaccharides
Glycerol
Carbon
Cellulose
Biosynthesis
Algae
Nucleotides
Fatty acids
Metabolism
Principal component analysis
Glucose
Amino acids
Rhodophyta
Cells
Derivatives
Plant Cells

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Bioengineering

Cite this

Global metabolite profiling of agarose degradation by Saccharophagus degradans 2-40. / Shin, Min Hye; Lee, Do Yup; Wohlgemuth, Gert; Choi, In-Geol; Fiehn, Oliver; Kim, Kyoung Heon.

In: New Biotechnology, Vol. 27, No. 2, 31.05.2010, p. 156-168.

Research output: Contribution to journalArticle

Shin, Min Hye ; Lee, Do Yup ; Wohlgemuth, Gert ; Choi, In-Geol ; Fiehn, Oliver ; Kim, Kyoung Heon. / Global metabolite profiling of agarose degradation by Saccharophagus degradans 2-40. In: New Biotechnology. 2010 ; Vol. 27, No. 2. pp. 156-168.
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