Global metabolite profiling of synovial fluid for the specific diagnosis of rheumatoid arthritis from other inflammatory arthritis

Sooah Kim, Jiwon Hwang, Jinhua Xuan, Young Hoon Jung, Hoon Suk Cha, Kyoung Heon Kim

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Currently, reliable biomarkers that can be used to distinguish rheumatoid arthritis (RA) from other inflammatory diseases are unavailable. To find possible distinctive metabolic patterns and biomarker candidates for RA, we performed global metabolite profiling of synovial fluid samples. Synovial fluid samples from 38 patients with RA, ankylosing spondylitis, Behçet's disease, and gout were analyzed by gas chromatography/time-of-flight mass spectrometry (GC/TOF MS). Orthogonal partial least-squares discriminant and hierarchical clustering analyses were performed for the discrimination of RA and non-RA groups. Variable importance for projection values were determined, and the Wilcoxon-Mann-Whitney test and the breakdown and one-way analysis of variance were conducted to identify potential biomarkers for RA. A total of 105 metabolites were identified from synovial fluid samples. The score plot of orthogonal partial least squares discriminant analysis showed significant discrimination between the RA and non-RA groups. The 20 metabolites, including citrulline, succinate, glutamine, octadecanol, isopalmitic acid, and glycerol, were identified as potential biomarkers for RA. These metabolites were found to be associated with the urea and TCA cycles as well as fatty acid and amino acid metabolism. The metabolomic analysis results demonstrated that global metabolite profiling by GC/TOF MS might be a useful tool for the effective diagnosis and further understanding of RA.

Original languageEnglish
Article numbere97501
JournalPLoS One
Volume9
Issue number6
DOIs
Publication statusPublished - 2014 Jun 2

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synovial fluid
rheumatoid arthritis
Synovial Fluid
arthritis
Metabolites
Arthritis
Biomarkers
Rheumatoid Arthritis
metabolites
Fluids
biomarkers
Gas chromatography
Mass spectrometry
Least-Squares Analysis
Citrulline
Gas Chromatography
least squares
Succinic Acid
Discriminant analysis
Mass Spectrometry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Global metabolite profiling of synovial fluid for the specific diagnosis of rheumatoid arthritis from other inflammatory arthritis. / Kim, Sooah; Hwang, Jiwon; Xuan, Jinhua; Jung, Young Hoon; Cha, Hoon Suk; Kim, Kyoung Heon.

In: PLoS One, Vol. 9, No. 6, e97501, 02.06.2014.

Research output: Contribution to journalArticle

Kim, Sooah ; Hwang, Jiwon ; Xuan, Jinhua ; Jung, Young Hoon ; Cha, Hoon Suk ; Kim, Kyoung Heon. / Global metabolite profiling of synovial fluid for the specific diagnosis of rheumatoid arthritis from other inflammatory arthritis. In: PLoS One. 2014 ; Vol. 9, No. 6.
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