Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae

Hisanori Tamaki, Cheol-Won Yun, Tomohiro Mizutani, Takahiro Tsuzuki, Yukinobu Takagi, Makiko Shinozaki, Yukiko Kodama, Katsuhiko Shirahige, Hidehiko Kumagai

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In the yeast, Saccharomyces cerevisiae, cell size is affected by the kind of carbon source in the medium. Here, we present evidence that the Gpr1 receptor and Gpa2 Gα subunit are required for both maintenance and modulation of cell size in response to glucose. In the presence of glucose, mutants lacking GPR1 or GPA2 gene showed smaller cells than the wild-type strain. Physiological studies revealed that protein synthesis rate was reduced in the mutant strains indicating that reduced growth rate, while the level of mRNAs for CLN1, 2 and 3 was not affected in all strains. Gene chip analysis also revealed a down-regulation in the expression of genes related to biosynthesis of not only protein but also other cellular component in the mutant strains. We also show that GPR1 and GPA2 are required for a rapid increase in cell size in response to glucose. Wild-type cells grown in ethanol quickly increased in size by addition of glucose, while little change was observed in the mutant strains, in which glucose-dependent cell cycle arrest caused by CLN1 repression was somewhat alleviated. Our study indicates that the yeast G-protein coupled receptor system consisting of Gpr1 and Gpa2 regulates cell size by affecting both growth rate and cell division.

Original languageEnglish
Pages (from-to)193-206
Number of pages14
JournalGenes to Cells
Volume10
Issue number3
DOIs
Publication statusPublished - 2005 Mar 1

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G-Protein-Coupled Receptors
Cell Size
Saccharomyces cerevisiae
Yeasts
Glucose
Fungal Proteins
Growth
Cell Cycle Checkpoints
Oligonucleotide Array Sequence Analysis
Cell Division
Proteins
Ethanol
Carbon
Down-Regulation
Maintenance
Gene Expression
Messenger RNA
Genes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae. / Tamaki, Hisanori; Yun, Cheol-Won; Mizutani, Tomohiro; Tsuzuki, Takahiro; Takagi, Yukinobu; Shinozaki, Makiko; Kodama, Yukiko; Shirahige, Katsuhiko; Kumagai, Hidehiko.

In: Genes to Cells, Vol. 10, No. 3, 01.03.2005, p. 193-206.

Research output: Contribution to journalArticle

Tamaki, H, Yun, C-W, Mizutani, T, Tsuzuki, T, Takagi, Y, Shinozaki, M, Kodama, Y, Shirahige, K & Kumagai, H 2005, 'Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae', Genes to Cells, vol. 10, no. 3, pp. 193-206. https://doi.org/10.1111/j.1365-2443.2005.00828.x
Tamaki, Hisanori ; Yun, Cheol-Won ; Mizutani, Tomohiro ; Tsuzuki, Takahiro ; Takagi, Yukinobu ; Shinozaki, Makiko ; Kodama, Yukiko ; Shirahige, Katsuhiko ; Kumagai, Hidehiko. / Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae. In: Genes to Cells. 2005 ; Vol. 10, No. 3. pp. 193-206.
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