Glucose deprivation decreases nitric oxide production via NADPH depletion in immunostimulated rat primary astrocytes

Young Shin Chan, Woong Choi Ji, Ryun Ryu Jae, Ho Ko Kwang, Jung Jin Choi, Hyun Soo Kim, Hee Sun Kim, Jae Chul Lee, Sun Jung Lee, Chun Kim Hyoung, Won Ki Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have previously reported that the production of nitric oxide (NO) in immunostimulated astrocytes was markedly decreased under glucose-deprived conditions. The present study was undertaken to find the contributing factor(s) for the decreased NO production in glucose-deprived immunostimulated astrocytes. NO production in rat primary astrocytes was stimulated for 24-48 h by cotreatment with lipopolysaccharides (1 μg/ml) and interferon-γ (100 U/ml). Decreased NO production in immunostimulated astrocytes by glucose deprivation was mimicked by the glycolytic inhibitor 2-deoxyglucose and reversed by addition of pyruvate and lactate. Glucose deprivation did not alter the expression of inducible nitric oxide synthase (iNOS) in immunostimulated astrocytes. Addition of β-NADPH, but not tetrahydrobiopterine, both of which are essential cofactors for NOS function, completely restored the NO production that was decreased in glucose-deprived immunostimulated astrocytes. Glucose deprivation and immunostimulation synergistically reduced intracellular NADPH level in astrocytes. The results indicate that glucose deprivation decreases NO production in immunostimulated astrocytes by depleting intracellular NADPH, a cofactor of iNOS.

Original languageEnglish
Pages (from-to)268-274
Number of pages7
JournalGLIA
Volume37
Issue number3
DOIs
Publication statusPublished - 2002 Mar 1
Externally publishedYes

Keywords

  • Cell death
  • Cofactor
  • Glutathione
  • Tetrahydrobiopterine
  • iNOS

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Chan, Y. S., Ji, W. C., Jae, R. R., Kwang, H. K., Choi, J. J., Kim, H. S., Kim, H. S., Lee, J. C., Lee, S. J., Hyoung, C. K., & Kim, W. K. (2002). Glucose deprivation decreases nitric oxide production via NADPH depletion in immunostimulated rat primary astrocytes. GLIA, 37(3), 268-274. https://doi.org/10.1002/glia.10032