TY - JOUR
T1 - Glucose oxidase-copper hybrid nanoflowers embedded with magnetic nanoparticles as an effective antibacterial agent
AU - Lee, Inseon
AU - Cheon, Hong Jae
AU - Adhikari, Manab Deb
AU - Tran, Tai Duc
AU - Yeon, Kyung Min
AU - Kim, Moon Il
AU - Kim, Jungbae
N1 - Funding Information:
This work was supported by the Global Research Laboratory Program ( 2014K1A1A2043032 ) through the National Research Foundation of Korea (NRF) grants funded by the Korea government Ministry of Science and ICT. This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT (NRF- 2017R1C1B2009460 )). This work was also supported by a Korea University Grant. Appendix A
Funding Information:
This work was supported by the Global Research Laboratory Program (2014K1A1A2043032) through the National Research Foundation of Korea (NRF) grants funded by the Korea government Ministry of Science and ICT. This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT (NRF- 2017R1C1B2009460)). This work was also supported by a Korea University Grant.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/7/15
Y1 - 2020/7/15
N2 - Bacterial contamination causes various problems ranging from bacterial infection to biofouling. As an effective and non-toxic agent for bacterial de-contamination, glucose oxidase (GOx)-copper hybrid nanoflowers embedded with amine-functionalized magnetic nanoparticles (NH2-MNPs), called ‘MNP-GOx NFs’, are developed. Positively-charged NH2-MNPs and negatively-charged GOx molecules are first interacted via electrostatic attraction which can be controlled by changing the buffer pH, and the follow-up addition of copper(II) sulfate leads to blooming of nanoflowers (MNP-GOx NFs) after incubation at room temperature for 3 days. MNP-GOx NFs show effective antibacterial activity by generating H2O2 from GOx-catalyzed glucose oxidation. For example, 99.9% killings of Staphylococcus aureus and Escherichia coli are achieved after 3 h treatment of 106/mL cells with 0.2 and 3.0 mg/mL MNP-GOx NFs, respectively, revealing that Gram-positive S. aureus with mono-layer membrane system is more vulnerable to the treatment of MNP-GOx NFs than Gram-negative E. coli with two-layer membrane system. MNP-GOx NFs can maintain 97% of bactericidal activity even after recycled uses by magnetic separation for eight times iterative bacterial killings. Finally, MNP-GOx NFs are employed for the fabrication of antibacterial gauzes. MNP-GOx NFs have also opened up a great potential for their applications in biosensors, biofuel cells and bioconversion as well as bacterial de-contamination.
AB - Bacterial contamination causes various problems ranging from bacterial infection to biofouling. As an effective and non-toxic agent for bacterial de-contamination, glucose oxidase (GOx)-copper hybrid nanoflowers embedded with amine-functionalized magnetic nanoparticles (NH2-MNPs), called ‘MNP-GOx NFs’, are developed. Positively-charged NH2-MNPs and negatively-charged GOx molecules are first interacted via electrostatic attraction which can be controlled by changing the buffer pH, and the follow-up addition of copper(II) sulfate leads to blooming of nanoflowers (MNP-GOx NFs) after incubation at room temperature for 3 days. MNP-GOx NFs show effective antibacterial activity by generating H2O2 from GOx-catalyzed glucose oxidation. For example, 99.9% killings of Staphylococcus aureus and Escherichia coli are achieved after 3 h treatment of 106/mL cells with 0.2 and 3.0 mg/mL MNP-GOx NFs, respectively, revealing that Gram-positive S. aureus with mono-layer membrane system is more vulnerable to the treatment of MNP-GOx NFs than Gram-negative E. coli with two-layer membrane system. MNP-GOx NFs can maintain 97% of bactericidal activity even after recycled uses by magnetic separation for eight times iterative bacterial killings. Finally, MNP-GOx NFs are employed for the fabrication of antibacterial gauzes. MNP-GOx NFs have also opened up a great potential for their applications in biosensors, biofuel cells and bioconversion as well as bacterial de-contamination.
KW - Antibacterial activity
KW - Enzyme-inorganic hybrid nanoflowers
KW - Glucose oxidase
UR - http://www.scopus.com/inward/record.url?scp=85075899016&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2019.11.129
DO - 10.1016/j.ijbiomac.2019.11.129
M3 - Article
C2 - 31751699
AN - SCOPUS:85075899016
VL - 155
SP - 1520
EP - 1531
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
ER -