Abstract
Arabidopsis hexokinase1 (HXK1) is a glucose sensor that regulates gene expression and plant growth and development. We have previously developed a high glucose (6%) assay based on the seedling developmental arrest to isolate and characterize the glucose-insensitive (gin) mutants. The analysis of gin2 as a null HXK1 mutant has revealed that the regulatory functions of HXK1 are distinct from its conventional role in glycolysis. In the Nov 3rd issue of Cell, we presented a new insight into the mechanism of HXK1-dependent glucose signaling. By combining proteomic and binary interaction screens, we discover two HXK1 unconventional partners (HUPs). HXK1 and HUPs form a core complex in the nucleus and directly regulate glucose-responsive gene expression and plant growth. As the 6% glucose assay is complicated by additional osmotic stress and nitrate signals, we have tested the gin2 and hup mutants using the 2% glucose assay. We believe that the new and more physiological glucose assay could help us better dissect the molecular mechanisms that link glucose regulation to diverse plant signaling pathways. Further functional analysis of gin mutants and the components in the novel nuclear HXK1 complex will provide more comprehensive mechanistic understanding of glucose sensing and signaling in plants.
| Original language | English |
|---|---|
| Pages (from-to) | 123-124 |
| Number of pages | 2 |
| Journal | Plant Signaling and Behavior |
| Volume | 2 |
| Issue number | 2 |
| Publication status | Published - 2007 Mar 1 |
| Externally published | Yes |
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Keywords
- Glucose sensing and signaling
- Glucose-insensitive mutants
- Hexokinase1 complex
- HXK1 unconventional partners
ASJC Scopus subject areas
- Plant Science
Cite this
Glucose signaling through nuclear hexokinase1 complex in Arabidopsis. / Cho, Young Hee; Yoo, Sang-Dong; Sheen, Jen.
In: Plant Signaling and Behavior, Vol. 2, No. 2, 01.03.2007, p. 123-124.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glucose signaling through nuclear hexokinase1 complex in Arabidopsis
AU - Cho, Young Hee
AU - Yoo, Sang-Dong
AU - Sheen, Jen
PY - 2007/3/1
Y1 - 2007/3/1
N2 - Arabidopsis hexokinase1 (HXK1) is a glucose sensor that regulates gene expression and plant growth and development. We have previously developed a high glucose (6%) assay based on the seedling developmental arrest to isolate and characterize the glucose-insensitive (gin) mutants. The analysis of gin2 as a null HXK1 mutant has revealed that the regulatory functions of HXK1 are distinct from its conventional role in glycolysis. In the Nov 3rd issue of Cell, we presented a new insight into the mechanism of HXK1-dependent glucose signaling. By combining proteomic and binary interaction screens, we discover two HXK1 unconventional partners (HUPs). HXK1 and HUPs form a core complex in the nucleus and directly regulate glucose-responsive gene expression and plant growth. As the 6% glucose assay is complicated by additional osmotic stress and nitrate signals, we have tested the gin2 and hup mutants using the 2% glucose assay. We believe that the new and more physiological glucose assay could help us better dissect the molecular mechanisms that link glucose regulation to diverse plant signaling pathways. Further functional analysis of gin mutants and the components in the novel nuclear HXK1 complex will provide more comprehensive mechanistic understanding of glucose sensing and signaling in plants.
AB - Arabidopsis hexokinase1 (HXK1) is a glucose sensor that regulates gene expression and plant growth and development. We have previously developed a high glucose (6%) assay based on the seedling developmental arrest to isolate and characterize the glucose-insensitive (gin) mutants. The analysis of gin2 as a null HXK1 mutant has revealed that the regulatory functions of HXK1 are distinct from its conventional role in glycolysis. In the Nov 3rd issue of Cell, we presented a new insight into the mechanism of HXK1-dependent glucose signaling. By combining proteomic and binary interaction screens, we discover two HXK1 unconventional partners (HUPs). HXK1 and HUPs form a core complex in the nucleus and directly regulate glucose-responsive gene expression and plant growth. As the 6% glucose assay is complicated by additional osmotic stress and nitrate signals, we have tested the gin2 and hup mutants using the 2% glucose assay. We believe that the new and more physiological glucose assay could help us better dissect the molecular mechanisms that link glucose regulation to diverse plant signaling pathways. Further functional analysis of gin mutants and the components in the novel nuclear HXK1 complex will provide more comprehensive mechanistic understanding of glucose sensing and signaling in plants.
KW - Glucose sensing and signaling
KW - Glucose-insensitive mutants
KW - Hexokinase1 complex
KW - HXK1 unconventional partners
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M3 - Article
VL - 2
SP - 123
EP - 124
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
SN - 1559-2316
IS - 2
ER -