Glutamate decarboxylase-derived IDDM autoantigens displayed on self-assembled protein nanoparticles

Hyoung Choi, Ji Young Ahn, Sang Jun Sim, Jeewon Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The recombinant ferritin heavy chain (FTN-H) formed self-assembled spherical nanoparticles with the size comparable to native one. We tried to express the GAD65 COOH-terminal fragments, i.e., 448-585 (GAD65 448-585), 487-585 (GAD65 487-585), and 512-585 (GAD65 512-585) amino acid fragments, using FTN-H as N-terminus fusion expression partner in Escherichia coli. All of recombinant fusion proteins (FTN-H::GAD65 448-585, FTN-H::GAD65 487-585, and FTN-H::GAD65 512-585) also formed spherical nanoparticles due probably to the self-assembly function of the fused ferritin heavy chain. The antigenic epitopes within GAD65 448-585, GAD65 487-585, and GAD65 512-585 against insulin-dependent diabetes mellitus (IDDM) marker (autoantibodies against GAD65) were localized at the surface of the spherical protein nanoparticles so that anti-GAD65 Ab could recognize them. Protein nanoparticles like FTN-H seem to provide distinct advantages over other inorganic nanoparticles (e.g., Au, Ag, CdSe, etc.) in that through the bacterial synthesis, the active capture probes can be located at the nanoparticle surface with constant orientation/conformation via covalent cross-linking without complex chemistry. Also it is possible for the protein nanoparticles to have uniform particle size, which is rarely achieved in the chemical synthesis of inorganic nanoparticles. Thus, the recombinant ferritin particles can be used as a three-dimensional (spherical) and nanometer-scale probe structure that is a key component in ultra-sensitive protein chip for detecting protein-small molecule interactions and protein-protein interactions.

Original languageEnglish
Pages (from-to)604-608
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume327
Issue number2
DOIs
Publication statusPublished - 2005 Feb 11

Fingerprint

Apoferritins
Glutamate Decarboxylase
Autoantigens
Medical problems
Type 1 Diabetes Mellitus
Nanoparticles
Insulin
Proteins
Inorganic Chemicals
Recombinant Fusion Proteins
Synthetic Chemistry Techniques
Protein Array Analysis
Ferritins
Particle Size
Crystal orientation
Autoantibodies
Self assembly
Escherichia coli
Conformations
Epitopes

Keywords

  • Active capture probe
  • Constant orientation
  • IDDM marker
  • Self-assembled protein nanoparticles
  • Three-dimensional and nanometer-scale probe structure
  • Uniform size

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Glutamate decarboxylase-derived IDDM autoantigens displayed on self-assembled protein nanoparticles. / Choi, Hyoung; Ahn, Ji Young; Sim, Sang Jun; Lee, Jeewon.

In: Biochemical and Biophysical Research Communications, Vol. 327, No. 2, 11.02.2005, p. 604-608.

Research output: Contribution to journalArticle

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