Glutathione dimerization-based plasmonic nanoswitch for biodetection of reactive oxygen and nitrogen species

Sumit Kumar, Won Kyu Rhim, Dong Kwon Lim, Jwa Min Nam

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Reactive oxygen and nitrogen species (ROS and RNS) are continuously produced in the cellular systems and are controlled by several antioxidant mechanisms. Here, we developed a straightforward, sensitive, and quantitative assay for the colorimetric and spectroscopic detection of various ROS and RNS such as H2O2, ·OH, -OCl, NO·, and O2- using glutathione-modified gold nanoparticles (GSH-AuNPs). A basic principle here is that the GSHs on the AuNP surface can be readily detached via the formation of glutathione disulfides upon the addition of ROS and RNS, and destabilized particles can aggregate to generate the plasmonic couplings between plasmonic AuNPs that trigger the red shift in UV-vis spectrum and solution color change. For nonradical species such as H 2O2, this process can be more efficiently achieved by converting them into radical species via the Fenton reaction. Using this strategy, we were able to rapidly and quantitatively distinguish among cancerous and normal cells based on ROS and RNS production.

Original languageEnglish
Pages (from-to)2221-2230
Number of pages10
JournalACS nano
Volume7
Issue number3
DOIs
Publication statusPublished - 2013 Mar 26
Externally publishedYes

Keywords

  • Fenton reaction
  • glutathione
  • plasmonic nanoparticle
  • reactive nitrogen species
  • reactive oxygen species

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Glutathione dimerization-based plasmonic nanoswitch for biodetection of reactive oxygen and nitrogen species'. Together they form a unique fingerprint.

  • Cite this