Gold, poly(β-amino ester) nanoparticles for small interfering RNA delivery

Jae Seung Lee, Jordan J. Green, Kevin T. Love, Joel Sunshine, Robert Langer, Daniel G. Anderson

Research output: Contribution to journalArticle

229 Citations (Scopus)

Abstract

The safe and effective delivery of RNA therapeutics remains the major barrier to their broad clinical application. Here we develop a new nanoparticulate delivery system based on inorganic particles and biodegradable polycations. First, gold nanoparticles were modified with the hydrophilic polymer poly(ethylene glycol) (PEG), and then small interfering RNA (siRNA) was conjugated to the nanoparticles via biodegradable disulfide linkages, with 30 strands of siRNA per nanoparticle. The particles were then coated with a library of end-modified poly(β-amino ester)s (PBAEs), previously identified as capable of facilitating intracellular DNA delivery. Nanoparticulate formulations developed here facilitate high levels of in vitro siRNA delivery, facilitating delivery as good or better than the commercially available lipid reagent, Lipofectamine 2000.

Original languageEnglish
Pages (from-to)2402-2406
Number of pages5
JournalNano Letters
Volume9
Issue number6
DOIs
Publication statusPublished - 2009 Jun 10

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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    Lee, J. S., Green, J. J., Love, K. T., Sunshine, J., Langer, R., & Anderson, D. G. (2009). Gold, poly(β-amino ester) nanoparticles for small interfering RNA delivery. Nano Letters, 9(6), 2402-2406. https://doi.org/10.1021/nl9009793