Gpr1, a putative G-protein-coupled receptor, regulates morphogenesis and hypha formation in the pathogenic fungus Candida albicans

Takuya Miwa, Yukinobu Takagi, Makiko Shinozaki, Cheol-Won Yun, Wiley A. Schell, John R. Perfect, Hidehiko Kumagai, Hisanori Tamaki

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

In response to various extracellular signals, the morphology of the human fungal pathogen Candida albicans switches from yeast to hypha form. Here, we report that GPR1 encoding a putative G-protein-coupled receptor and GPA2 encoding a Ga subunit are required for hypha formation and morphogenesis in C. albicans. Mutants lacking Gpr1 (gpr1/gpr1) or Gpa2 (gpa2/gpa2) are defective in hypha formation and morphogenesis on solid hypha-inducing media. These phenotypic defects in solid cultures are suppressed by exogenously added dibutyryl-cyclic AMP (dibutyryl-cAMP). Biochemical studies also reveal that GPR1 and GPA2 are required for a glucose-dependent increase in cellular cAMP. An epistasis analysis indicates that Gpr1 functions upstream of Gpa2 in the same signaling pathway, and a two-hybrid assay reveals that the carboxyl-terminal tail of Gpr1 interacts with Gpa2. Moreover, expression levels of HWP1 and ECE1, which are cAMP-dependent hypha-specific genes, are reduced in both mutant strains. These findings support a model that Gpr1, as well as Gpa2, regulates hypha formation and morphogenesis in a cAMP-dependent manner. In contrast, GPR1 and GPA2 are not required for hypha formation in liquid fetal bovine serum (FBS) medium. Furthermore, the gpr1 and the gpa2 mutant strains are fully virulent in a mouse infection. These findings suggest that Gpr1 and Gpa2 are involved in the glucose-sensing machinery that regulates morphogenesis and hypha formation in solid media via a cAMP-dependeni mechanism, but they are not required for hypha formation in liquid medium or during invasive candidiasis.

Original languageEnglish
Pages (from-to)919-931
Number of pages13
JournalEukaryotic Cell
Volume3
Issue number4
DOIs
Publication statusPublished - 2004 Aug 1

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Hyphae
G-Protein-Coupled Receptors
Candida albicans
Morphogenesis
hyphae
morphogenesis
Fungi
fungi
mutants
Invasive Candidiasis
G-protein coupled receptors
Glucose
two hybrid system techniques
Bucladesine
Two-Hybrid System Techniques
glucose
candidiasis
liquids
epistasis
fetal bovine serum

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Gpr1, a putative G-protein-coupled receptor, regulates morphogenesis and hypha formation in the pathogenic fungus Candida albicans. / Miwa, Takuya; Takagi, Yukinobu; Shinozaki, Makiko; Yun, Cheol-Won; Schell, Wiley A.; Perfect, John R.; Kumagai, Hidehiko; Tamaki, Hisanori.

In: Eukaryotic Cell, Vol. 3, No. 4, 01.08.2004, p. 919-931.

Research output: Contribution to journalArticle

Miwa, Takuya ; Takagi, Yukinobu ; Shinozaki, Makiko ; Yun, Cheol-Won ; Schell, Wiley A. ; Perfect, John R. ; Kumagai, Hidehiko ; Tamaki, Hisanori. / Gpr1, a putative G-protein-coupled receptor, regulates morphogenesis and hypha formation in the pathogenic fungus Candida albicans. In: Eukaryotic Cell. 2004 ; Vol. 3, No. 4. pp. 919-931.
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