GPR1 regulates filamentous growth through FLO11 in yeast Saccharomyces cerevisiae

Hisanori Tamaki, Takuya Miwa, Makiko Shinozaki, Megumi Saito, Cheol-Won Yun, Kenji Yamamoto, Hidehiko Kumagai

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Cell growth and differentiation are regulated by nutrient availability in the yeast Saccharomyces cerevisiae. Under conditions of nitrogen limitation, diploid cells of S. cerevisiae differentiate to a filamentous growth known as a pseudohyphal growth, while haploid cells produce invasive filaments which penetrate the agar in nutrient-rich medium. We have found that GPR1, which encodes a putative G-protein-coupled receptor, is required for both pseudohyphal and invasive growth. Pseudohyphal growth was defective in Δgpr1/Δgpr1 mutant strain and this defect was reversed by addition of cAMP. Also, haploid Δgpr1 mutant strain was defective in invasive growth. Northern blot analysis revealed that the transcriptional level of FLO11, which encodes a recently identified cell surface flocculin required for pseudohyphal growth, was reduced in Δgpr1 mutant strain. These results indicate that GPR1 regulates both pseudohyphal and invasive growth by a cAMP-dependent mechanism. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)164-168
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume267
Issue number1
DOIs
Publication statusPublished - 2000 Jan 7
Externally publishedYes

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Yeast
Saccharomyces cerevisiae
Yeasts
Growth
Haploidy
Nutrients
Food
Cell growth
G-Protein-Coupled Receptors
Agar
Diploidy
Northern Blotting
Nitrogen
Cell Differentiation
Availability
Defects

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

GPR1 regulates filamentous growth through FLO11 in yeast Saccharomyces cerevisiae. / Tamaki, Hisanori; Miwa, Takuya; Shinozaki, Makiko; Saito, Megumi; Yun, Cheol-Won; Yamamoto, Kenji; Kumagai, Hidehiko.

In: Biochemical and Biophysical Research Communications, Vol. 267, No. 1, 07.01.2000, p. 164-168.

Research output: Contribution to journalArticle

Tamaki, Hisanori ; Miwa, Takuya ; Shinozaki, Makiko ; Saito, Megumi ; Yun, Cheol-Won ; Yamamoto, Kenji ; Kumagai, Hidehiko. / GPR1 regulates filamentous growth through FLO11 in yeast Saccharomyces cerevisiae. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 267, No. 1. pp. 164-168.
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