Abstract
A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0144T was isolated from the seawater of South Sea, Korea. The 16S rRNA gene sequence was found to share the highest sequence similarity to Gramella sediminilitoris GHTF-27T (97.7 %) and the strain branched within the radiation of the genus Gramella in phylogenetic trees. Thus, the taxonomic position of the novel isolate was investigated using a polyphasic approach and complete genome sequencing. Strain LPB0144T has a circular chromosome of 2.98 Mb with DNA G+C content of 38.2 mol%. The genome includes 2604 protein-coding genes and three copies of rRNA operons. The detected respiratory quinone (MK-6) and the major polar lipid (phosphatidylethanolamine) resemble the chemotaxonomic profile of other Gramella species. The major cellular fatty acid profile (iso-C15: 0, iso-C16: 0, iso-C17: 0 3-OH and anteiso-C15: 0) is also within the range of Gramella, but detailed composition and amounts were found to be different from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Gramella. On the basis of polyphasic taxonomic data obtained here, we propose strain LPB0144T as a novel Gramella species, for which the name Gramella salexigens sp. nov. is proposed. The type strain is LPB0144T (=KACC 18894T=JCM 31560T).
| Original language | English |
|---|---|
| Article number | 002850 |
| Pages (from-to) | 2381-2385 |
| Number of pages | 5 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 68 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2018 Jul 1 |
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Keywords
- Genome
- Gmella salexigens
- Gramella
- Novel species
ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Gramella salexigens sp. Nov., isolated from seawater. / Shin, Su Kyoung; Kim, Eunji; Yi, Hana.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 7, 002850, 01.07.2018, p. 2381-2385.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gramella salexigens sp. Nov., isolated from seawater
AU - Shin, Su Kyoung
AU - Kim, Eunji
AU - Yi, Hana
PY - 2018/7/1
Y1 - 2018/7/1
N2 - A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0144T was isolated from the seawater of South Sea, Korea. The 16S rRNA gene sequence was found to share the highest sequence similarity to Gramella sediminilitoris GHTF-27T (97.7 %) and the strain branched within the radiation of the genus Gramella in phylogenetic trees. Thus, the taxonomic position of the novel isolate was investigated using a polyphasic approach and complete genome sequencing. Strain LPB0144T has a circular chromosome of 2.98 Mb with DNA G+C content of 38.2 mol%. The genome includes 2604 protein-coding genes and three copies of rRNA operons. The detected respiratory quinone (MK-6) and the major polar lipid (phosphatidylethanolamine) resemble the chemotaxonomic profile of other Gramella species. The major cellular fatty acid profile (iso-C15: 0, iso-C16: 0, iso-C17: 0 3-OH and anteiso-C15: 0) is also within the range of Gramella, but detailed composition and amounts were found to be different from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Gramella. On the basis of polyphasic taxonomic data obtained here, we propose strain LPB0144T as a novel Gramella species, for which the name Gramella salexigens sp. nov. is proposed. The type strain is LPB0144T (=KACC 18894T=JCM 31560T).
AB - A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0144T was isolated from the seawater of South Sea, Korea. The 16S rRNA gene sequence was found to share the highest sequence similarity to Gramella sediminilitoris GHTF-27T (97.7 %) and the strain branched within the radiation of the genus Gramella in phylogenetic trees. Thus, the taxonomic position of the novel isolate was investigated using a polyphasic approach and complete genome sequencing. Strain LPB0144T has a circular chromosome of 2.98 Mb with DNA G+C content of 38.2 mol%. The genome includes 2604 protein-coding genes and three copies of rRNA operons. The detected respiratory quinone (MK-6) and the major polar lipid (phosphatidylethanolamine) resemble the chemotaxonomic profile of other Gramella species. The major cellular fatty acid profile (iso-C15: 0, iso-C16: 0, iso-C17: 0 3-OH and anteiso-C15: 0) is also within the range of Gramella, but detailed composition and amounts were found to be different from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Gramella. On the basis of polyphasic taxonomic data obtained here, we propose strain LPB0144T as a novel Gramella species, for which the name Gramella salexigens sp. nov. is proposed. The type strain is LPB0144T (=KACC 18894T=JCM 31560T).
KW - Genome
KW - Gmella salexigens
KW - Gramella
KW - Novel species
UR - http://www.scopus.com/inward/record.url?scp=85049915097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049915097&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002850
DO - 10.1099/ijsem.0.002850
M3 - Article
VL - 68
SP - 2381
EP - 2385
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
SN - 1466-5026
IS - 7
M1 - 002850
ER -