Abstract
In this work, the effects of granular activated carbon (GAC) supplementation on hydrogen fermentation were investigated in Clostridium butyricum. Maximum hydrogen production rate and ultimate hydrogen volume increased up to 1.7 and 1.9 fold, respectively, with 1 g/L GAC supplementation. Indicators of stable hydrogen production, such as acetate and butyrate formation increased with increasing GAC concentration. To identify the factors for hydrogen production enhancement, transcriptome analysis was conducted. Functional genes related to hydrogen production increased by GAC supplementation (1.75 fold for pfor and 1.48 fold for oxct). On the other hand, functional genes related to hydrogen consumption decreased (1.78 fold for ldh, 0.67 fold for adh, 1.64 fold for hbd, 2.4 fold for crt, and 0.71 fold for buk). Considered together, these results suggested that GAC supplementation increased biohydrogen production by changing the metabolic flux associated with hydrogen production and consumption pathways.
| Original language | English |
|---|---|
| Pages (from-to) | 318-325 |
| Number of pages | 8 |
| Journal | Bioresource Technology |
| DOIs | |
| Publication status | Published - 2019 Jun 1 |
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Keywords
- Biohydrogen
- Clostridium butyricum
- Granular activated carbon (GAC)
- Metabolic pathway
- RNA sequencing
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal
Cite this
Granular activated carbon supplementation alters the metabolic flux of Clostridium butyricum for enhanced biohydrogen production. / Park, Jeong Hoon; Kim, Do Hyung; Kim, Han Shin; Wells, George F.; Park, Hee-Deung.
In: Bioresource Technology, 01.06.2019, p. 318-325.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Granular activated carbon supplementation alters the metabolic flux of Clostridium butyricum for enhanced biohydrogen production
AU - Park, Jeong Hoon
AU - Kim, Do Hyung
AU - Kim, Han Shin
AU - Wells, George F.
AU - Park, Hee-Deung
PY - 2019/6/1
Y1 - 2019/6/1
N2 - In this work, the effects of granular activated carbon (GAC) supplementation on hydrogen fermentation were investigated in Clostridium butyricum. Maximum hydrogen production rate and ultimate hydrogen volume increased up to 1.7 and 1.9 fold, respectively, with 1 g/L GAC supplementation. Indicators of stable hydrogen production, such as acetate and butyrate formation increased with increasing GAC concentration. To identify the factors for hydrogen production enhancement, transcriptome analysis was conducted. Functional genes related to hydrogen production increased by GAC supplementation (1.75 fold for pfor and 1.48 fold for oxct). On the other hand, functional genes related to hydrogen consumption decreased (1.78 fold for ldh, 0.67 fold for adh, 1.64 fold for hbd, 2.4 fold for crt, and 0.71 fold for buk). Considered together, these results suggested that GAC supplementation increased biohydrogen production by changing the metabolic flux associated with hydrogen production and consumption pathways.
AB - In this work, the effects of granular activated carbon (GAC) supplementation on hydrogen fermentation were investigated in Clostridium butyricum. Maximum hydrogen production rate and ultimate hydrogen volume increased up to 1.7 and 1.9 fold, respectively, with 1 g/L GAC supplementation. Indicators of stable hydrogen production, such as acetate and butyrate formation increased with increasing GAC concentration. To identify the factors for hydrogen production enhancement, transcriptome analysis was conducted. Functional genes related to hydrogen production increased by GAC supplementation (1.75 fold for pfor and 1.48 fold for oxct). On the other hand, functional genes related to hydrogen consumption decreased (1.78 fold for ldh, 0.67 fold for adh, 1.64 fold for hbd, 2.4 fold for crt, and 0.71 fold for buk). Considered together, these results suggested that GAC supplementation increased biohydrogen production by changing the metabolic flux associated with hydrogen production and consumption pathways.
KW - Biohydrogen
KW - Clostridium butyricum
KW - Granular activated carbon (GAC)
KW - Metabolic pathway
KW - RNA sequencing
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UR - http://www.scopus.com/inward/citedby.url?scp=85062727225&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2019.02.090
DO - 10.1016/j.biortech.2019.02.090
M3 - Article
C2 - 30826518
AN - SCOPUS:85062727225
SP - 318
EP - 325
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -