Granular activated carbon supplementation alters the metabolic flux of Clostridium butyricum for enhanced biohydrogen production

Jeong Hoon Park, Do Hyung Kim, Han Shin Kim, George F. Wells, Hee-Deung Park

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In this work, the effects of granular activated carbon (GAC) supplementation on hydrogen fermentation were investigated in Clostridium butyricum. Maximum hydrogen production rate and ultimate hydrogen volume increased up to 1.7 and 1.9 fold, respectively, with 1 g/L GAC supplementation. Indicators of stable hydrogen production, such as acetate and butyrate formation increased with increasing GAC concentration. To identify the factors for hydrogen production enhancement, transcriptome analysis was conducted. Functional genes related to hydrogen production increased by GAC supplementation (1.75 fold for pfor and 1.48 fold for oxct). On the other hand, functional genes related to hydrogen consumption decreased (1.78 fold for ldh, 0.67 fold for adh, 1.64 fold for hbd, 2.4 fold for crt, and 0.71 fold for buk). Considered together, these results suggested that GAC supplementation increased biohydrogen production by changing the metabolic flux associated with hydrogen production and consumption pathways.

Original languageEnglish
Pages (from-to)318-325
Number of pages8
JournalBioresource Technology
DOIs
Publication statusPublished - 2019 Jun 1

Keywords

  • Biohydrogen
  • Clostridium butyricum
  • Granular activated carbon (GAC)
  • Metabolic pathway
  • RNA sequencing

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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