Graphene-based field effect transistor enzymatic glucose biosensor using silk protein for enzyme immobilization and device substrate

Xueqiu You, James Jungho Pak

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

A silk fibroin-encapsulated graphene field effect transistor (FET) enzymatic biosensor that utilizes silk protein as both device substrate and enzyme immobilization material was developed for glucose detection. This biosensor detected glucose levels by measuring the differential drain-source current and the Dirac point shift of the graphene transistor as the glucose is oxidized by glucose oxidase that was immobilized in silk fibroin film on the graphene FET. The fabricated biosensors showed 0.1-10 mM large linear detection range, which covers the reference range of medical examination for diabetes diagnostics. The detection limit of the fabricated biosensors was approximately 0.1 mM (S/N = 3) with excellent selectivity, and the average sensitivity was 2.5 μA/mM measured at Vds = 100 mV and Vg = 0 V. Because this fibroin-encapsulated graphene FET enzymatic biosensor is biocompatible, flexible, and long-term stable, it holds a great promise for portable, wearable, and implantable continuous glucose monitoring applications.

Original languageEnglish
Pages (from-to)1357-1365
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume202
DOIs
Publication statusPublished - 2014 Oct 31

Fingerprint

Enzyme immobilization
silk
Silk
Graphite
Field effect transistors
immobilization
bioinstrumentation
Biosensors
glucose
Graphene
Glucose
enzymes
Fibroins
graphene
field effect transistors
proteins
Proteins
Substrates
Glucose Oxidase
Glucose oxidase

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Instrumentation

Cite this

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abstract = "A silk fibroin-encapsulated graphene field effect transistor (FET) enzymatic biosensor that utilizes silk protein as both device substrate and enzyme immobilization material was developed for glucose detection. This biosensor detected glucose levels by measuring the differential drain-source current and the Dirac point shift of the graphene transistor as the glucose is oxidized by glucose oxidase that was immobilized in silk fibroin film on the graphene FET. The fabricated biosensors showed 0.1-10 mM large linear detection range, which covers the reference range of medical examination for diabetes diagnostics. The detection limit of the fabricated biosensors was approximately 0.1 mM (S/N = 3) with excellent selectivity, and the average sensitivity was 2.5 μA/mM measured at Vds = 100 mV and Vg = 0 V. Because this fibroin-encapsulated graphene FET enzymatic biosensor is biocompatible, flexible, and long-term stable, it holds a great promise for portable, wearable, and implantable continuous glucose monitoring applications.",
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AU - You, Xueqiu

AU - Pak, James Jungho

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N2 - A silk fibroin-encapsulated graphene field effect transistor (FET) enzymatic biosensor that utilizes silk protein as both device substrate and enzyme immobilization material was developed for glucose detection. This biosensor detected glucose levels by measuring the differential drain-source current and the Dirac point shift of the graphene transistor as the glucose is oxidized by glucose oxidase that was immobilized in silk fibroin film on the graphene FET. The fabricated biosensors showed 0.1-10 mM large linear detection range, which covers the reference range of medical examination for diabetes diagnostics. The detection limit of the fabricated biosensors was approximately 0.1 mM (S/N = 3) with excellent selectivity, and the average sensitivity was 2.5 μA/mM measured at Vds = 100 mV and Vg = 0 V. Because this fibroin-encapsulated graphene FET enzymatic biosensor is biocompatible, flexible, and long-term stable, it holds a great promise for portable, wearable, and implantable continuous glucose monitoring applications.

AB - A silk fibroin-encapsulated graphene field effect transistor (FET) enzymatic biosensor that utilizes silk protein as both device substrate and enzyme immobilization material was developed for glucose detection. This biosensor detected glucose levels by measuring the differential drain-source current and the Dirac point shift of the graphene transistor as the glucose is oxidized by glucose oxidase that was immobilized in silk fibroin film on the graphene FET. The fabricated biosensors showed 0.1-10 mM large linear detection range, which covers the reference range of medical examination for diabetes diagnostics. The detection limit of the fabricated biosensors was approximately 0.1 mM (S/N = 3) with excellent selectivity, and the average sensitivity was 2.5 μA/mM measured at Vds = 100 mV and Vg = 0 V. Because this fibroin-encapsulated graphene FET enzymatic biosensor is biocompatible, flexible, and long-term stable, it holds a great promise for portable, wearable, and implantable continuous glucose monitoring applications.

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KW - Silk protein

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