Graphene-oxide quenching-based molecular beacon imaging of exosome-mediated transfer of neurogenic miR-193a on microfluidic platform

Hyun Jeong Oh, Jaehoon Kim, Hyejin Park, Seok Chung, Do Won Hwang, Dong Soo Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Graphene-oxide (GO) quenching-based molecular beacon was developed for rapid and sensitive detection of RNAs in living cells and tissues. Here, we applied GO quenching-based molecular beacon sensor to visualize neurogenic miR-193a levels delivered via exosomes during cell-non-autonomous neurogenesis in neural progenitor cells on a microfluidic platform. Exosomal transport was visualized using CD63-RFP plasmid vector, and FAM-labeled peptide nucleic acid (PNA) probe for the miR-193 sequence was designed to detect endogenous miR-193 expression. Fluorescence signals of FAM-PNA193a-GO were recovered in dibutyryl-cAMP-induced F11 cells, resulting from increased expression of miR-193a after neuronal differentiation. We observed delivery of miR-193a-containing exosomes released from differentiated donor F11 cells to recipient undifferentiated F11 cells. Fluorescence recovery was evident in exosome-stimulated recipient individual F11 cells in the microfluidic system. We propose molecular beacon imaging using PNA-GO complex for visualization of individual cellular expression of mature microRNAs. This system reveals the precise spatial localization and temporal sequences of mature miRNAs by intercellular exosomal delivery of messages for processes such as cell-non-autonomous neurogenesis.

Original languageEnglish
Pages (from-to)647-656
Number of pages10
JournalBiosensors and Bioelectronics
Volume126
DOIs
Publication statusPublished - 2019 Feb 1

Fingerprint

Exosomes
Molecular imaging
Molecular Imaging
Microfluidics
Graphite
Oxides
Graphene
Quenching
Peptide Nucleic Acids
MicroRNAs
Fluorescence
Nucleic Acid Probes
Neurogenesis
RNA
Plasmids
Visualization
Cells
Tissue
Recovery
Sensors

Keywords

  • Exosome-mediated neurogenesis
  • Graphene oxide (GO)
  • Microfluidic platform
  • miRNA-193a
  • Molecular beacon sensor
  • Peptide nucleic acid (PNA)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Graphene-oxide quenching-based molecular beacon imaging of exosome-mediated transfer of neurogenic miR-193a on microfluidic platform. / Oh, Hyun Jeong; Kim, Jaehoon; Park, Hyejin; Chung, Seok; Hwang, Do Won; Lee, Dong Soo.

In: Biosensors and Bioelectronics, Vol. 126, 01.02.2019, p. 647-656.

Research output: Contribution to journalArticle

Oh, Hyun Jeong ; Kim, Jaehoon ; Park, Hyejin ; Chung, Seok ; Hwang, Do Won ; Lee, Dong Soo. / Graphene-oxide quenching-based molecular beacon imaging of exosome-mediated transfer of neurogenic miR-193a on microfluidic platform. In: Biosensors and Bioelectronics. 2019 ; Vol. 126. pp. 647-656.
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