Green-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength

Ji Eun Jeong, Mohammad Afsar Uddin, Hwa Sook Ryu, Hee Chang Kim, Minsu Kang, Joonyoung Francis Joung, Sungnam Park, Sang Hee Shim, Han Young Woo

Research output: Contribution to journalArticlepeer-review

Abstract

We report newly synthesized fluorescence resonance energy transfer (FRET)-based green-, red-, and near-infrared (NIR)-emitting polymer dot (Pdot) probes. Fluorescent Pdots (∼60 nm) were prepared with a green-emissive conjugated polymer (PPDT-P, donor) alone or mixing the donor with a red- or NIR-emitting fluorophore (T-DCS or ITIC, acceptor), where an optically inert matrix polymer [poly(styrene-co-maleic anhydride)] was mixed together to minimize the aggregation-caused quenching by diluting the fluorophores and surface functionalization for further bioconjugation with antibodies for active targeting. Highly fluorescent green-emissive PPDT-P Pdots were prepared with a photoluminescence (PL) quantum efficiency of ∼30% and the FRET-mediated red and NIR PL was intensified by 5.3-8.5 times (with high FRET ratios of ∼9) via the efficient energy transfer (FRET efficiency of 80-98%) and antenna effect, compared with the signals obtained via direct excitation of the fluorophores in Pdots. All three types of Pdot/antibody conjugates were simultaneously immunostained to COS-7 cells (showing minimal cross-reactivity), reducing the tedious sequential immunostaining process to a single step. Finally, we obtained high-contrast three-color cell images with little spectral leakthrough by exciting all the probes simultaneously at a single wavelength at 405 nm without the need for a complicated or expensive multiple-excitation setup.

Original languageEnglish
Pages (from-to)6685-6696
Number of pages12
JournalChemistry of Materials
Volume32
Issue number15
DOIs
Publication statusPublished - 2020 Aug 11

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

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