Green tea polyphenol epigallocatechin-3-gallate suppresses collagen production and proliferation in keloid fibroblasts via inhibition of the STAT3-signaling pathway

Gyuman Park, Byung Sun Yoon, Jai Hee Moon, Bona Kim, Eun Kyoung Jun, Sejong Oh, Hyunggee Kim, Hea Joon Song, Joo Young Noh, Chil Hwan Oh, Seungkwon You

Research output: Contribution to journalArticle

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Abstract

Keloids are benign skin tumors characterized by collagen accumulation and hyperproliferation of fibroblasts. To find an effective therapy for keloids, we explored the pharmacological potential of (-)-epigallocatechin-3-gallate (EGCG), a widely investigated tumor-preventive agent. When applied to normal and keloid fibroblasts (KFs) in vitro, proliferation and migration of KFs were more strongly suppressed by EGCG than normal fibroblast proliferation and migration (IC50: 54.4 μM (keloid fibroblast (KF)) versus 63.0 μM (NF)). The level of Smad2/3, signal transducer and activator of transcription-3 (STAT3), and p38 phosphorylation is more enhanced in KFs, and EGCG inhibited phosphorylation of phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated protein kinase 1/2 (ERK1/2), and STAT3 (Tyr705 and Ser727). To evaluate the contribution of these pathways to keloid pathology, we treated KFs with specific inhibitors for PI3K, ERK1/2, or STAT3. Although a PI3K inhibitor significantly suppressed proliferation, PI3K and MEK/ERK inhibitors had a minor effect on migration and collagen production. However, a JAK2/STAT3 inhibitor and a STAT3 siRNA strongly suppressed proliferation, migration, and collagen production by KFs. We also found that treatment with EGCG suppressed growth and collagen production in the in vivo keloid model. This study demonstrates that EGCG suppresses the pathological characteristics of keloids through inhibition of the STAT3-signaling pathway. We propose that EGCG has potential in the treatment and prevention of keloids.

Original languageEnglish
Pages (from-to)2429-2441
Number of pages13
JournalJournal of Investigative Dermatology
Volume128
Issue number10
DOIs
Publication statusPublished - 2008 Oct 1

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Keloid
STAT3 Transcription Factor
Polyphenols
Tea
Fibroblasts
Collagen
Phosphatidylinositol 3-Kinase
Phosphorylation
Protein Kinases
Tumors
Mitogen-Activated Protein Kinase 3
epigallocatechin gallate
Mitogen-Activated Protein Kinase Kinases
Pathology
Small Interfering RNA
Skin
Inhibitory Concentration 50
Neoplasms

ASJC Scopus subject areas

  • Dermatology

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Green tea polyphenol epigallocatechin-3-gallate suppresses collagen production and proliferation in keloid fibroblasts via inhibition of the STAT3-signaling pathway. / Park, Gyuman; Yoon, Byung Sun; Moon, Jai Hee; Kim, Bona; Jun, Eun Kyoung; Oh, Sejong; Kim, Hyunggee; Song, Hea Joon; Noh, Joo Young; Oh, Chil Hwan; You, Seungkwon.

In: Journal of Investigative Dermatology, Vol. 128, No. 10, 01.10.2008, p. 2429-2441.

Research output: Contribution to journalArticle

Park, Gyuman ; Yoon, Byung Sun ; Moon, Jai Hee ; Kim, Bona ; Jun, Eun Kyoung ; Oh, Sejong ; Kim, Hyunggee ; Song, Hea Joon ; Noh, Joo Young ; Oh, Chil Hwan ; You, Seungkwon. / Green tea polyphenol epigallocatechin-3-gallate suppresses collagen production and proliferation in keloid fibroblasts via inhibition of the STAT3-signaling pathway. In: Journal of Investigative Dermatology. 2008 ; Vol. 128, No. 10. pp. 2429-2441.
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T1 - Green tea polyphenol epigallocatechin-3-gallate suppresses collagen production and proliferation in keloid fibroblasts via inhibition of the STAT3-signaling pathway

AU - Park, Gyuman

AU - Yoon, Byung Sun

AU - Moon, Jai Hee

AU - Kim, Bona

AU - Jun, Eun Kyoung

AU - Oh, Sejong

AU - Kim, Hyunggee

AU - Song, Hea Joon

AU - Noh, Joo Young

AU - Oh, Chil Hwan

AU - You, Seungkwon

PY - 2008/10/1

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N2 - Keloids are benign skin tumors characterized by collagen accumulation and hyperproliferation of fibroblasts. To find an effective therapy for keloids, we explored the pharmacological potential of (-)-epigallocatechin-3-gallate (EGCG), a widely investigated tumor-preventive agent. When applied to normal and keloid fibroblasts (KFs) in vitro, proliferation and migration of KFs were more strongly suppressed by EGCG than normal fibroblast proliferation and migration (IC50: 54.4 μM (keloid fibroblast (KF)) versus 63.0 μM (NF)). The level of Smad2/3, signal transducer and activator of transcription-3 (STAT3), and p38 phosphorylation is more enhanced in KFs, and EGCG inhibited phosphorylation of phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated protein kinase 1/2 (ERK1/2), and STAT3 (Tyr705 and Ser727). To evaluate the contribution of these pathways to keloid pathology, we treated KFs with specific inhibitors for PI3K, ERK1/2, or STAT3. Although a PI3K inhibitor significantly suppressed proliferation, PI3K and MEK/ERK inhibitors had a minor effect on migration and collagen production. However, a JAK2/STAT3 inhibitor and a STAT3 siRNA strongly suppressed proliferation, migration, and collagen production by KFs. We also found that treatment with EGCG suppressed growth and collagen production in the in vivo keloid model. This study demonstrates that EGCG suppresses the pathological characteristics of keloids through inhibition of the STAT3-signaling pathway. We propose that EGCG has potential in the treatment and prevention of keloids.

AB - Keloids are benign skin tumors characterized by collagen accumulation and hyperproliferation of fibroblasts. To find an effective therapy for keloids, we explored the pharmacological potential of (-)-epigallocatechin-3-gallate (EGCG), a widely investigated tumor-preventive agent. When applied to normal and keloid fibroblasts (KFs) in vitro, proliferation and migration of KFs were more strongly suppressed by EGCG than normal fibroblast proliferation and migration (IC50: 54.4 μM (keloid fibroblast (KF)) versus 63.0 μM (NF)). The level of Smad2/3, signal transducer and activator of transcription-3 (STAT3), and p38 phosphorylation is more enhanced in KFs, and EGCG inhibited phosphorylation of phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated protein kinase 1/2 (ERK1/2), and STAT3 (Tyr705 and Ser727). To evaluate the contribution of these pathways to keloid pathology, we treated KFs with specific inhibitors for PI3K, ERK1/2, or STAT3. Although a PI3K inhibitor significantly suppressed proliferation, PI3K and MEK/ERK inhibitors had a minor effect on migration and collagen production. However, a JAK2/STAT3 inhibitor and a STAT3 siRNA strongly suppressed proliferation, migration, and collagen production by KFs. We also found that treatment with EGCG suppressed growth and collagen production in the in vivo keloid model. This study demonstrates that EGCG suppresses the pathological characteristics of keloids through inhibition of the STAT3-signaling pathway. We propose that EGCG has potential in the treatment and prevention of keloids.

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