Abstract
This paper reports the effects of changing pH (5-7) and temperature (T, 40-60 °C) on the efficiencies of bacterial hydrolysis of suspended organic matter (SOM) in wastewater from food waste recycling (FWR) and the changes in the bacterial community responsible for this hydrolysis. Maximum hydrolysis efficiency (i.e., 50.5% reduction of volatile suspended solids) was predicted to occur at pH 5.7 and T=44.5 °C. Changes in short-chain volatile organic acid profiles and in acidogenic bacterial communities were investigated under these conditions. Propionic and butyric acids concentrations increased rapidly during the first 2 days of incubation. Several band sequences consistent with Clostridium spp. were detected using denaturing gel gradient electrophoresis. Clostridium thermopalmarium and Clostridium novyi seemed to contribute to butyric acid production during the first 1.5 days of acidification of FWR wastewater, and C. thermopalmarium was a major butyric acid producer afterward. C. novyi was an important propionic acid producer. These two species appear to be important contributors to hydrolysis of SOM in the wastewater. Other acidogenic anaerobes, Aeromonas sharmana, Bacillus coagulans, and Pseudomonas plecoglossicida, were also indentified.
| Original language | English |
|---|---|
| Pages (from-to) | 1611-1618 |
| Number of pages | 8 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 85 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2010 Feb 1 |
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Keywords
- Acidogen
- Denaturing gradient gel electrophoresis
- Food waste-recycling wastewater
- Hydrolysis
- Microbial community structure
- Particulate organic materials
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Growth condition and bacterial community for maximum hydrolysis of suspended organic materials in anaerobic digestion of food waste-recycling wastewater. / Kim, Man Deok; Song, Minkyung; Jo, Minho; Shin, Seung Gu; Khim, Jeehyeong; Hwang, Seokhwan.
In: Applied Microbiology and Biotechnology, Vol. 85, No. 5, 01.02.2010, p. 1611-1618.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth condition and bacterial community for maximum hydrolysis of suspended organic materials in anaerobic digestion of food waste-recycling wastewater
AU - Kim, Man Deok
AU - Song, Minkyung
AU - Jo, Minho
AU - Shin, Seung Gu
AU - Khim, Jeehyeong
AU - Hwang, Seokhwan
PY - 2010/2/1
Y1 - 2010/2/1
N2 - This paper reports the effects of changing pH (5-7) and temperature (T, 40-60 °C) on the efficiencies of bacterial hydrolysis of suspended organic matter (SOM) in wastewater from food waste recycling (FWR) and the changes in the bacterial community responsible for this hydrolysis. Maximum hydrolysis efficiency (i.e., 50.5% reduction of volatile suspended solids) was predicted to occur at pH 5.7 and T=44.5 °C. Changes in short-chain volatile organic acid profiles and in acidogenic bacterial communities were investigated under these conditions. Propionic and butyric acids concentrations increased rapidly during the first 2 days of incubation. Several band sequences consistent with Clostridium spp. were detected using denaturing gel gradient electrophoresis. Clostridium thermopalmarium and Clostridium novyi seemed to contribute to butyric acid production during the first 1.5 days of acidification of FWR wastewater, and C. thermopalmarium was a major butyric acid producer afterward. C. novyi was an important propionic acid producer. These two species appear to be important contributors to hydrolysis of SOM in the wastewater. Other acidogenic anaerobes, Aeromonas sharmana, Bacillus coagulans, and Pseudomonas plecoglossicida, were also indentified.
AB - This paper reports the effects of changing pH (5-7) and temperature (T, 40-60 °C) on the efficiencies of bacterial hydrolysis of suspended organic matter (SOM) in wastewater from food waste recycling (FWR) and the changes in the bacterial community responsible for this hydrolysis. Maximum hydrolysis efficiency (i.e., 50.5% reduction of volatile suspended solids) was predicted to occur at pH 5.7 and T=44.5 °C. Changes in short-chain volatile organic acid profiles and in acidogenic bacterial communities were investigated under these conditions. Propionic and butyric acids concentrations increased rapidly during the first 2 days of incubation. Several band sequences consistent with Clostridium spp. were detected using denaturing gel gradient electrophoresis. Clostridium thermopalmarium and Clostridium novyi seemed to contribute to butyric acid production during the first 1.5 days of acidification of FWR wastewater, and C. thermopalmarium was a major butyric acid producer afterward. C. novyi was an important propionic acid producer. These two species appear to be important contributors to hydrolysis of SOM in the wastewater. Other acidogenic anaerobes, Aeromonas sharmana, Bacillus coagulans, and Pseudomonas plecoglossicida, were also indentified.
KW - Acidogen
KW - Denaturing gradient gel electrophoresis
KW - Food waste-recycling wastewater
KW - Hydrolysis
KW - Microbial community structure
KW - Particulate organic materials
UR - http://www.scopus.com/inward/record.url?scp=76649110200&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76649110200&partnerID=8YFLogxK
U2 - 10.1007/s00253-009-2316-x
DO - 10.1007/s00253-009-2316-x
M3 - Article
C2 - 19894044
AN - SCOPUS:76649110200
VL - 85
SP - 1611
EP - 1618
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -