Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization

Dong Hoon Choi, Sung Nam Kang, Seong Min Kim, Samy Gobaa, Bang Ju Park, Ik Hwan Kim, Yoon Ki Joung, Dong Keun Han

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.

Original languageEnglish
Pages (from-to)602-610
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume141
DOIs
Publication statusPublished - 2016 May 1

Fingerprint

heparins
Hyaluronic acid
Stents
Hyaluronic Acid
Heparin
Intercellular Signaling Peptides and Proteins
induction
acids
sulfones
Ethyldimethylaminopropyl Carbodiimide
Coatings
Hepatocyte Growth Factor
Gap Junctions
Linings
Vascular Endothelial Growth Factor A
Restoration
endothelium
Endothelium
thrombosis
Blood Vessels

Keywords

  • Dopamine
  • Growth factor
  • Heparin
  • Hyaluronic acid
  • Re-endothelialization
  • Stent

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization. / Choi, Dong Hoon; Kang, Sung Nam; Kim, Seong Min; Gobaa, Samy; Park, Bang Ju; Kim, Ik Hwan; Joung, Yoon Ki; Han, Dong Keun.

In: Colloids and Surfaces B: Biointerfaces, Vol. 141, 01.05.2016, p. 602-610.

Research output: Contribution to journalArticle

Choi, Dong Hoon ; Kang, Sung Nam ; Kim, Seong Min ; Gobaa, Samy ; Park, Bang Ju ; Kim, Ik Hwan ; Joung, Yoon Ki ; Han, Dong Keun. / Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization. In: Colloids and Surfaces B: Biointerfaces. 2016 ; Vol. 141. pp. 602-610.
@article{9bbf94b6e9d64b82813c2ee15e7aacea,
title = "Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization",
abstract = "Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.",
keywords = "Dopamine, Growth factor, Heparin, Hyaluronic acid, Re-endothelialization, Stent",
author = "Choi, {Dong Hoon} and Kang, {Sung Nam} and Kim, {Seong Min} and Samy Gobaa and Park, {Bang Ju} and Kim, {Ik Hwan} and Joung, {Yoon Ki} and Han, {Dong Keun}",
year = "2016",
month = "5",
day = "1",
doi = "10.1016/j.colsurfb.2016.01.028",
language = "English",
volume = "141",
pages = "602--610",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

TY - JOUR

T1 - Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization

AU - Choi, Dong Hoon

AU - Kang, Sung Nam

AU - Kim, Seong Min

AU - Gobaa, Samy

AU - Park, Bang Ju

AU - Kim, Ik Hwan

AU - Joung, Yoon Ki

AU - Han, Dong Keun

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.

AB - Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.

KW - Dopamine

KW - Growth factor

KW - Heparin

KW - Hyaluronic acid

KW - Re-endothelialization

KW - Stent

UR - http://www.scopus.com/inward/record.url?scp=84959148135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959148135&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2016.01.028

DO - 10.1016/j.colsurfb.2016.01.028

M3 - Article

VL - 141

SP - 602

EP - 610

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -