Guanosine 5',3'-cyclic monophosphate enhances lipopolysaccharide-induced nitric oxide synthase expression in mixed glial cell cultures of rat

Sang-Hyun Choi, Kyung-Ho Shin, Sang Wook Kang, Yeon Sook Chun, Boe Gwun Chun

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7 Citations (Scopus)


Primary mixed glial cell cultures treated with lipopolysaccharide (LPS; 1.0 μg/ml) showed biphasic increases of inducible nitric oxide synthase (iNOS) mRNA expression 6 h and 24-36 h after LPS treatment. Dibutyryl-guanosine 5',3'-cyclic monophosphate (db-cGMP; 1.0 mM) enhanced the second phase of the LPS-induced iNOS expression 24 and 30 h after LPS stimulation. KT5823 (1.0 μM), a protein kinase G (PKG) inhibitor, inhibited the LPS-induced iNOS expressions at 24 and 30 h and their enhancements caused by db-cGMP. In astrocyte-enriched cultures with reduced microglial contamination, the LPS-induced iNOS expression was decreased, though slightly enhanced by db-cGMP. These results suggest that cGMP/PKG signaling may be involved in the second phase of the LPS-induced glial iNOS expression and its upregulation, which are apparent in the presence of microglial cells. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)29-32
Number of pages4
JournalNeuroscience Letters
Issue number1
Publication statusPublished - 1999 Nov 26



  • Astrocytes
  • Guanosine 5',3'-cyclic monophosphate
  • Inducible nitric oxide synthase
  • Microglial cells
  • Nitric oxide
  • Protein kinase G

ASJC Scopus subject areas

  • Neuroscience(all)

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