Guggulsterone suppresses LPS induced inflammation of human middle ear epithelial cells (HMEEC)

Jae-Jun Song, Seong Keun Kwon, Chang Gun Cho, Seok Won Park, Sungwon Chae

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: Guggulsterone is a bioactive constituent of resinous sap originating from the guggul tree, Commiphora mukul, which has been used over several thousands of years to treat various diseases, including atherosclerosis, rheumatism, and obesity. However, the effect of guggulsterone inflammatory reactions induced by lipopolysaccharide (LSP) is not known. The aim of this study was to evaluate the anti-inflammatory effect of guggulsterone on cultured human middle ear epithelial cells (HMEEC). Methods: The effect of guggulsterone on LPS-induced tumor necrosis factor-alpha (TNF-α) and cyclooxygenase-2 (COX-2) expression was evaluated in HMEEC by real-time reverse transcription polymerase chain reaction (RT-PCR). LPS-induced COX-2 production and degradation of the inhibitor kB-alpha (IkB-α) were determined by Western blot analysis. Results: Guggulsterone significantly inhibited LPS-induced upregulation of TNF-α and COX-2 in a dose-dependent manner. COX-2 protein production by LPS was significantly suppressed by the guggulsterone pretreatment. Furthermore, LPS-induced IkB-α degradation was suppressed by the guggulsterone pretreatment. Conclusions: These results show that the guggulsterone has inhibitory effect on TNF-α expression and COX-2 production and it may be mediated through its inhibition of nuclear factor-kB activation. Our findings provide an insight into the molecular mechanisms underlying the anti-inflammatory activities of guggulsterone in relationship to otitis media.

Original languageEnglish
Pages (from-to)1384-1387
Number of pages4
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume74
Issue number12
DOIs
Publication statusPublished - 2010 Dec 1

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Otitis Media
Epithelial Cells
Cyclooxygenase 2
Commiphora
Tumor Necrosis Factor-alpha
Middle Ear
Anti-Inflammatory Agents
pregna-4,17-diene-3,16-dione
Rheumatic Diseases
Reverse Transcription
Lipopolysaccharides
Atherosclerosis
Up-Regulation
Obesity
Western Blotting
Polymerase Chain Reaction

Keywords

  • Guggulsterone
  • NF-kappaB
  • Otitis media

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology

Cite this

Guggulsterone suppresses LPS induced inflammation of human middle ear epithelial cells (HMEEC). / Song, Jae-Jun; Kwon, Seong Keun; Cho, Chang Gun; Park, Seok Won; Chae, Sungwon.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 74, No. 12, 01.12.2010, p. 1384-1387.

Research output: Contribution to journalArticle

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N2 - Objective: Guggulsterone is a bioactive constituent of resinous sap originating from the guggul tree, Commiphora mukul, which has been used over several thousands of years to treat various diseases, including atherosclerosis, rheumatism, and obesity. However, the effect of guggulsterone inflammatory reactions induced by lipopolysaccharide (LSP) is not known. The aim of this study was to evaluate the anti-inflammatory effect of guggulsterone on cultured human middle ear epithelial cells (HMEEC). Methods: The effect of guggulsterone on LPS-induced tumor necrosis factor-alpha (TNF-α) and cyclooxygenase-2 (COX-2) expression was evaluated in HMEEC by real-time reverse transcription polymerase chain reaction (RT-PCR). LPS-induced COX-2 production and degradation of the inhibitor kB-alpha (IkB-α) were determined by Western blot analysis. Results: Guggulsterone significantly inhibited LPS-induced upregulation of TNF-α and COX-2 in a dose-dependent manner. COX-2 protein production by LPS was significantly suppressed by the guggulsterone pretreatment. Furthermore, LPS-induced IkB-α degradation was suppressed by the guggulsterone pretreatment. Conclusions: These results show that the guggulsterone has inhibitory effect on TNF-α expression and COX-2 production and it may be mediated through its inhibition of nuclear factor-kB activation. Our findings provide an insight into the molecular mechanisms underlying the anti-inflammatory activities of guggulsterone in relationship to otitis media.

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