Guided three-dimensional growth of functional cardiomyocytes on polyethylene glycol nanostructures

Deok Ho Kim, Pilnam Kim, Inseon Song, Jae Min Cha, Sang Ho Lee, Byungkyu Kim, Kahp Y. Suh

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

We introduce well-defined nanopillar arrays of a poly(ethylene glycol) (PEG) hydrogel as a cell culture platform to guide a 3D construct of primary rat cardiomyocytes in vitro for potential tissue engineering applications. Ultraviolet (UV)-assisted capillary lithography was used to fabricate highly uniform ∼150 nm PEG pillars with ∼400 nm height. It was found that cell adhesion was significantly enhanced on PEG nanopillars (132 ± 29 cells/mm 2) compared to that on the bare PEG control (39 ± 17 cells/mm 2) (p < 0.05) but substantially reduced compared to that on the glass control (502 ± 45 cells/mm2) (p < 0.01). Furthermore, in colonizing cardiomyocytes, the nanopillars stimulated self-assembled aggregates among the contacting cells with 3D growth, which is a unique feature for nanopatterned PEG hydrogels as a cell culture substrate. The 3D-grown cardiomyocytes retained their conductive and contractile properties, as evidenced by the observation of beating cardiomyocytes with robust action potential generation.

Original languageEnglish
Pages (from-to)5419-5426
Number of pages8
JournalLangmuir
Volume22
Issue number12
DOIs
Publication statusPublished - 2006 Jun 6

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Nanostructures
Cardiac Myocytes
Polyethylene glycols
glycols
polyethylenes
Growth
cells
Cell Culture Techniques
Cell culture
Hydrogels
Ethylene Glycol
Hydrogel
Tissue Engineering
Cell Adhesion
tissue engineering
Action Potentials
Glass
Cell adhesion
Bioelectric potentials
Observation

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Guided three-dimensional growth of functional cardiomyocytes on polyethylene glycol nanostructures. / Kim, Deok Ho; Kim, Pilnam; Song, Inseon; Cha, Jae Min; Lee, Sang Ho; Kim, Byungkyu; Suh, Kahp Y.

In: Langmuir, Vol. 22, No. 12, 06.06.2006, p. 5419-5426.

Research output: Contribution to journalArticle

Kim, Deok Ho ; Kim, Pilnam ; Song, Inseon ; Cha, Jae Min ; Lee, Sang Ho ; Kim, Byungkyu ; Suh, Kahp Y. / Guided three-dimensional growth of functional cardiomyocytes on polyethylene glycol nanostructures. In: Langmuir. 2006 ; Vol. 22, No. 12. pp. 5419-5426.
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