Hair-growth stimulation by conditioned medium from vitamin D3-activated preadipocytes in C57BL/6 mice

Min Kyung Jung, Soogyeong Ha, Scarlett Yoona Huh, Seung Beom Park, Sangyoon Kim, Yoolhee Yang, Daejin Kim, Dae Young Hur, Hyuk Jeong, Sa Ik Bang, Hyunjeong Park, Dae Ho Cho

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aims Recently, immature adipocyte lineage cells have been suggested as a potential hair-growth stimulator. Diverse studies have been attempted to find methods for the preconditioning of immature adipocyte lineage cells. The present study investigates the effect of conditioned medium (CM) from vitamin D3 (Vd3) pre-activated preadipocytes on hair-growth ability. Main methods To test the effect of CM from Vd3 pre-activated preadipocytes on hair-growth efficiency in mice, we compared the differences in hair regenerated after injecting CM from mouse preadipocytes pre-activated with or without Vd3. Next, to determine the regulating factors, the VEGF level was measured by ELISA and angiogenesis level was evaluated by IHC. Finally, the signaling mechanism was investigated by inhibitor kinase assay and western blotting. Key findings The CM from Vd3 pre-activated preadipocyte injection markedly promoted the ability of hair regeneration in mice. The VEGF levels were increased by Vd3 treatment in vitro and the CM from Vd3 pre-activated preadipocytes significantly increased the angiogenesis in vivo, suggesting the involvement of angiognensis in the hair regeneration induced by CM from pre-activated preadipocytes. In signaling study, Vd3-enhanced VEGF production was reduced by an ERK1/2 inhibitor and the level of ERK1/2 phosphorylation was increased by treatment with Vd3. Significance This has been the first report on CM from Vd3 pre-activated preadipocyte displaying stimulatory effects on hair growth via the enhancement of angiogenesis in a hairless-induced C57BL/6 mice.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalLife Sciences
Volume128
DOIs
Publication statusPublished - 2015 May 1
Externally publishedYes

Fingerprint

Cholecalciferol
Conditioned Culture Medium
Inbred C57BL Mouse
Hair
Growth
Vascular Endothelial Growth Factor A
Adipocytes
Regeneration
Phosphorylation
Assays
Phosphotransferases
Western Blotting
Enzyme-Linked Immunosorbent Assay
Injections
Therapeutics

Keywords

  • Angiogenesis
  • Extracellular signal-regulated kinase 1/2
  • Hair growth
  • Preadipocyte
  • Vitamin D3

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Hair-growth stimulation by conditioned medium from vitamin D3-activated preadipocytes in C57BL/6 mice. / Jung, Min Kyung; Ha, Soogyeong; Huh, Scarlett Yoona; Park, Seung Beom; Kim, Sangyoon; Yang, Yoolhee; Kim, Daejin; Hur, Dae Young; Jeong, Hyuk; Bang, Sa Ik; Park, Hyunjeong; Cho, Dae Ho.

In: Life Sciences, Vol. 128, 01.05.2015, p. 39-46.

Research output: Contribution to journalArticle

Jung, MK, Ha, S, Huh, SY, Park, SB, Kim, S, Yang, Y, Kim, D, Hur, DY, Jeong, H, Bang, SI, Park, H & Cho, DH 2015, 'Hair-growth stimulation by conditioned medium from vitamin D3-activated preadipocytes in C57BL/6 mice', Life Sciences, vol. 128, pp. 39-46. https://doi.org/10.1016/j.lfs.2015.02.018
Jung, Min Kyung ; Ha, Soogyeong ; Huh, Scarlett Yoona ; Park, Seung Beom ; Kim, Sangyoon ; Yang, Yoolhee ; Kim, Daejin ; Hur, Dae Young ; Jeong, Hyuk ; Bang, Sa Ik ; Park, Hyunjeong ; Cho, Dae Ho. / Hair-growth stimulation by conditioned medium from vitamin D3-activated preadipocytes in C57BL/6 mice. In: Life Sciences. 2015 ; Vol. 128. pp. 39-46.
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abstract = "Aims Recently, immature adipocyte lineage cells have been suggested as a potential hair-growth stimulator. Diverse studies have been attempted to find methods for the preconditioning of immature adipocyte lineage cells. The present study investigates the effect of conditioned medium (CM) from vitamin D3 (Vd3) pre-activated preadipocytes on hair-growth ability. Main methods To test the effect of CM from Vd3 pre-activated preadipocytes on hair-growth efficiency in mice, we compared the differences in hair regenerated after injecting CM from mouse preadipocytes pre-activated with or without Vd3. Next, to determine the regulating factors, the VEGF level was measured by ELISA and angiogenesis level was evaluated by IHC. Finally, the signaling mechanism was investigated by inhibitor kinase assay and western blotting. Key findings The CM from Vd3 pre-activated preadipocyte injection markedly promoted the ability of hair regeneration in mice. The VEGF levels were increased by Vd3 treatment in vitro and the CM from Vd3 pre-activated preadipocytes significantly increased the angiogenesis in vivo, suggesting the involvement of angiognensis in the hair regeneration induced by CM from pre-activated preadipocytes. In signaling study, Vd3-enhanced VEGF production was reduced by an ERK1/2 inhibitor and the level of ERK1/2 phosphorylation was increased by treatment with Vd3. Significance This has been the first report on CM from Vd3 pre-activated preadipocyte displaying stimulatory effects on hair growth via the enhancement of angiogenesis in a hairless-induced C57BL/6 mice.",
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AU - Jung, Min Kyung

AU - Ha, Soogyeong

AU - Huh, Scarlett Yoona

AU - Park, Seung Beom

AU - Kim, Sangyoon

AU - Yang, Yoolhee

AU - Kim, Daejin

AU - Hur, Dae Young

AU - Jeong, Hyuk

AU - Bang, Sa Ik

AU - Park, Hyunjeong

AU - Cho, Dae Ho

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N2 - Aims Recently, immature adipocyte lineage cells have been suggested as a potential hair-growth stimulator. Diverse studies have been attempted to find methods for the preconditioning of immature adipocyte lineage cells. The present study investigates the effect of conditioned medium (CM) from vitamin D3 (Vd3) pre-activated preadipocytes on hair-growth ability. Main methods To test the effect of CM from Vd3 pre-activated preadipocytes on hair-growth efficiency in mice, we compared the differences in hair regenerated after injecting CM from mouse preadipocytes pre-activated with or without Vd3. Next, to determine the regulating factors, the VEGF level was measured by ELISA and angiogenesis level was evaluated by IHC. Finally, the signaling mechanism was investigated by inhibitor kinase assay and western blotting. Key findings The CM from Vd3 pre-activated preadipocyte injection markedly promoted the ability of hair regeneration in mice. The VEGF levels were increased by Vd3 treatment in vitro and the CM from Vd3 pre-activated preadipocytes significantly increased the angiogenesis in vivo, suggesting the involvement of angiognensis in the hair regeneration induced by CM from pre-activated preadipocytes. In signaling study, Vd3-enhanced VEGF production was reduced by an ERK1/2 inhibitor and the level of ERK1/2 phosphorylation was increased by treatment with Vd3. Significance This has been the first report on CM from Vd3 pre-activated preadipocyte displaying stimulatory effects on hair growth via the enhancement of angiogenesis in a hairless-induced C57BL/6 mice.

AB - Aims Recently, immature adipocyte lineage cells have been suggested as a potential hair-growth stimulator. Diverse studies have been attempted to find methods for the preconditioning of immature adipocyte lineage cells. The present study investigates the effect of conditioned medium (CM) from vitamin D3 (Vd3) pre-activated preadipocytes on hair-growth ability. Main methods To test the effect of CM from Vd3 pre-activated preadipocytes on hair-growth efficiency in mice, we compared the differences in hair regenerated after injecting CM from mouse preadipocytes pre-activated with or without Vd3. Next, to determine the regulating factors, the VEGF level was measured by ELISA and angiogenesis level was evaluated by IHC. Finally, the signaling mechanism was investigated by inhibitor kinase assay and western blotting. Key findings The CM from Vd3 pre-activated preadipocyte injection markedly promoted the ability of hair regeneration in mice. The VEGF levels were increased by Vd3 treatment in vitro and the CM from Vd3 pre-activated preadipocytes significantly increased the angiogenesis in vivo, suggesting the involvement of angiognensis in the hair regeneration induced by CM from pre-activated preadipocytes. In signaling study, Vd3-enhanced VEGF production was reduced by an ERK1/2 inhibitor and the level of ERK1/2 phosphorylation was increased by treatment with Vd3. Significance This has been the first report on CM from Vd3 pre-activated preadipocyte displaying stimulatory effects on hair growth via the enhancement of angiogenesis in a hairless-induced C57BL/6 mice.

KW - Angiogenesis

KW - Extracellular signal-regulated kinase 1/2

KW - Hair growth

KW - Preadipocyte

KW - Vitamin D3

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