Hantaan virus enters cells by clathrin-dependent receptor-mediated endocytosis

Mirim Jin, Junghyun Park, Sungwook Lee, Boyoun Park, Jinwook Shin, Ki-Joon Song, Tae In Ahn, Sue Yun Hwang, Byung-Yoon Ahn, Kwangseog Ahn

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110 Citations (Scopus)

Abstract

The cellular entry of Hantaan virus (HTN) occurs through interactions with β3 integrins as cellular receptors. However, the process of HTN infection following attachment to the cell surface is not well understood. Our data indicate that overexpression of a dominant-negative mutant dynamin inhibits HTN internalization and that compounds that block clathrin- but not caveolae-dependent endocytosis also reduce HTN infectivity. In addition, we show that HTN colocalizes with the clathrin heavy chain but not with caveolae. At the early phase of infection HTN colocalizes with EEA-1, an early endosome marker, and later, HTN colocalizes with LAMP-1, a lysosome marker. Cells treated with lysosomotropic agents are largely resistant to infection, suggesting that a low-pH-dependent step is required for HTN infection. These findings demonstrate that HTN enters cells via the clathrin-coated pit pathway and uses low-pH-dependent intracellular compartments for infectious entry.

Original languageEnglish
Pages (from-to)60-69
Number of pages10
JournalVirology
Volume294
Issue number1
DOIs
Publication statusPublished - 2002 Sep 2

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Keywords

  • Clathrin-dependent endocytosis
  • Endosome
  • Entry
  • Hantaan virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Jin, M., Park, J., Lee, S., Park, B., Shin, J., Song, K-J., Ahn, T. I., Hwang, S. Y., Ahn, B-Y., & Ahn, K. (2002). Hantaan virus enters cells by clathrin-dependent receptor-mediated endocytosis. Virology, 294(1), 60-69. https://doi.org/10.1006/viro.2001.1303