Hantaan virus nucleocapsid protein stimulates MDM2-dependent p53 degradation

Sun Whan Park, Myung Guk Han, Chan Park, Young Ran Ju, Byung-Yoon Ahn, Jungsang Ryou

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Apoptosis has been shown to be induced and downregulated by the Hantaan virus (HTNV) nucleocapsid (N) protein. To address these conflicting data, expression of the p53 protein, one of the key molecules involved in apoptosis, was assessed in the presence of the N protein in A549 and HeLa cells. The amount of p53, increased by drug treatment, was reduced when cells were infected with HTNV or transfected with an expression vector of the HTNV N protein. When cells were treated with a proteasome inhibitor (MG132) or an MDM2 antagonist (Nutlin-3), p53 expression was not reduced in N protein-overexpressed cells. We concluded that the HTNV N protein ubiquitinates and degrades p53 MDM2-dependently. Here we report downregulation of p53 expression through a post-translational mechanism: MDM2-dependent ubiquitination and degradation by the HTNV N protein. These results indicate that N protein-dependent p53 degradation through the ubiquitin proteasome system is one of the anti-apoptotic mechanisms employed by HTNV.

Original languageEnglish
Pages (from-to)2424-2428
Number of pages5
JournalJournal of General Virology
Volume94
Issue numberPART 11
DOIs
Publication statusPublished - 2013 Nov 1

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Hantaan virus
Proteins
Down-Regulation
Apoptosis
Proteasome Inhibitors
Ubiquitination
Proteasome Endopeptidase Complex
Ubiquitin
HeLa Cells
Hantaan virus nucleocapsid protein
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Virology

Cite this

Hantaan virus nucleocapsid protein stimulates MDM2-dependent p53 degradation. / Park, Sun Whan; Han, Myung Guk; Park, Chan; Ju, Young Ran; Ahn, Byung-Yoon; Ryou, Jungsang.

In: Journal of General Virology, Vol. 94, No. PART 11, 01.11.2013, p. 2424-2428.

Research output: Contribution to journalArticle

Park, Sun Whan ; Han, Myung Guk ; Park, Chan ; Ju, Young Ran ; Ahn, Byung-Yoon ; Ryou, Jungsang. / Hantaan virus nucleocapsid protein stimulates MDM2-dependent p53 degradation. In: Journal of General Virology. 2013 ; Vol. 94, No. PART 11. pp. 2424-2428.
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