Hantavirus surveillance and genetic diversity targeting small mammals at Camp Humphreys, a US military installation and new expansion site, Republic of Korea

Heung Chul Kim, Won Keun Kim, Terry A. Klein, Sung Tae Chong, Peter V. Nunn, Jeong Ah Kim, Seung Ho Lee, Jin Sun No, Jin Won Song

Research output: Contribution to journalArticle

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Abstract

Small mammal surveillance was conducted (2008±2010, 2012) at Camp (Cp) Humphreys, a US Army installation and new expansion site, Republic of Korea (ROK), to identify hemorrhagic fever with renal syndrome health threats to US military/civilian populations during its ongoing expansion phase. Small mammals were collected using Sherman live capture traps and transported to Korea University where they were euthanized, tissues removed, and assayed to determine hantavirus IgG antibody-positive and hantavirus-positive rates by RT-PCR. A total of 2,364 small mammals were captured over 11,300 trap nights (capture rate = 20.92%). Apodemus agrarius was the most commonly collected (76.65%), with capture rates of 9.62% and 21.70% for Cp Humphreys and the expansion site, respectively. Overall, Hantaan virus (HTNV) IgG antibody-positive (Ab+) rate for A. agrarius was 2.15% (39/1,812). A total of 5.43% (10/184) Crocidura lasiura, 0.79% (2/254) Microtus fortis and 2.44% (1/41) Micromys minutus were serologically IgG Ab+ for hantaviruses. HTNV-specific RT-PCR demonstrated that 28.2% (11/39) HTNV Ab+ A. agrarius harbored the 328-nt sequence of the GC glycoprotein-encoding M segment of HTNV. Among them, the whole genome sequences of 3 HTNV strains were obtained by conventional RT-PCR and Rapid Amplification cDNA Ends PCR. Phylogenetic analyses of the HTNV strains from Cp Humphreys and the expansion site, Pyeongtaek, show a greater diversity of rodent-borne hantaviruses compared to HTNV previously identified in Gyeonggi province of the ROK. Thus, this study provides significant insights for raising HFRS threat awareness, analysis, and risk reduction strategies in southern Gyeonggi province.

Original languageEnglish
Article numbere0176514
JournalPLoS One
Volume12
Issue number4
DOIs
Publication statusPublished - 2017 Apr 1

Fingerprint

Hantaan virus
military lands
Hantavirus
Republic of Korea
Mammals
South Korea
small mammals
Viruses
Apodemus agrarius
genetic variation
monitoring
Hemorrhagic Fever with Renal Syndrome
Polymerase Chain Reaction
Immunoglobulin G
reverse transcriptase polymerase chain reaction
traps
Crocidura
Murinae
Arvicolinae
antibodies

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hantavirus surveillance and genetic diversity targeting small mammals at Camp Humphreys, a US military installation and new expansion site, Republic of Korea. / Kim, Heung Chul; Kim, Won Keun; Klein, Terry A.; Chong, Sung Tae; Nunn, Peter V.; Kim, Jeong Ah; Lee, Seung Ho; No, Jin Sun; Song, Jin Won.

In: PLoS One, Vol. 12, No. 4, e0176514, 01.04.2017.

Research output: Contribution to journalArticle

Kim, Heung Chul ; Kim, Won Keun ; Klein, Terry A. ; Chong, Sung Tae ; Nunn, Peter V. ; Kim, Jeong Ah ; Lee, Seung Ho ; No, Jin Sun ; Song, Jin Won. / Hantavirus surveillance and genetic diversity targeting small mammals at Camp Humphreys, a US military installation and new expansion site, Republic of Korea. In: PLoS One. 2017 ; Vol. 12, No. 4.
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