Harnessing α-l-fucosidase forin vivocellular senescence imaging

Seyoung Koo, Miae Won, Hao Li, Won Young Kim, Mingle Li, Chenxu Yan, Amit Sharma, Zhiqian Guo, Wei Hong Zhu, Jonathan L. Sessler, Jin Yong Lee, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Precise detection of cellular senescence may allow its role in biological systems to be evaluated more effectively, while supporting studies of therapeutic candidates designed to evade its detrimental effect on physical function. We report here studies of α-l-fucosidase (α-fuc) as a biomarker for cellular senescence and the development of an α-fuc-responsive aggregation induced emission (AIE) probe, termedQM-NHαfucdesigned to complement more conventional probes based on β-galactosidase (β-gal). UsingQM-NHαfuc, the onset of replicative-, reactive oxygen species (ROS)-, ultraviolet A (UVA)-, and drug-induced senescence could be probed effectively.QM-NHαfucalso proved capable of identifying senescent cells lacking β-gal expression. The non-invasive real-time senescence tracking provided byQM-NHαfucwas validated in anin vivosenescence model. The results presented in this study lead us to suggest that theQM-NHαfuccould emerge as a useful tool for investigating senescence processes in biological systems.

Original languageEnglish
Pages (from-to)10054-10062
Number of pages9
JournalChemical Science
Volume12
Issue number29
DOIs
Publication statusPublished - 2021 Aug 7

ASJC Scopus subject areas

  • Chemistry(all)

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