Harnessing α-l-fucosidase forin vivocellular senescence imaging

Seyoung Koo; Miae Won; Hao Li; Won Young Kim; Mingle Li; Chenxu Yan; Amit Sharma; Zhiqian Guo; Wei Hong Zhu; Jonathan L. Sessler; Jin Yong Lee; Jong Seung Kim

doi:10.1039/d1sc02259h

Harnessing α-l-fucosidase forin vivocellular senescence imaging

Seyoung Koo, Miae Won, Hao Li, Won Young Kim, Mingle Li, Chenxu Yan, Amit Sharma, Zhiqian Guo, Wei Hong Zhu, Jonathan L. Sessler, Jin Yong Lee, Jong Seung Kim

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9 Citations (Scopus)

Abstract

Precise detection of cellular senescence may allow its role in biological systems to be evaluated more effectively, while supporting studies of therapeutic candidates designed to evade its detrimental effect on physical function. We report here studies of α-l-fucosidase (α-fuc) as a biomarker for cellular senescence and the development of an α-fuc-responsive aggregation induced emission (AIE) probe, termedQM-NHαfucdesigned to complement more conventional probes based on β-galactosidase (β-gal). UsingQM-NHαfuc, the onset of replicative-, reactive oxygen species (ROS)-, ultraviolet A (UVA)-, and drug-induced senescence could be probed effectively.QM-NHαfucalso proved capable of identifying senescent cells lacking β-gal expression. The non-invasive real-time senescence tracking provided byQM-NHαfucwas validated in anin vivosenescence model. The results presented in this study lead us to suggest that theQM-NHαfuccould emerge as a useful tool for investigating senescence processes in biological systems.

Original language	English
Pages (from-to)	10054-10062
Number of pages	9
Journal	Chemical Science
Volume	12
Issue number	29
DOIs	https://doi.org/10.1039/d1sc02259h
Publication status	Published - 2021 Aug 7

ASJC Scopus subject areas

Chemistry(all)

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@article{fb509b42e5914ff39607833e96a4a0e3,

title = "Harnessing α-l-fucosidase forin vivocellular senescence imaging",

abstract = "Precise detection of cellular senescence may allow its role in biological systems to be evaluated more effectively, while supporting studies of therapeutic candidates designed to evade its detrimental effect on physical function. We report here studies of α-l-fucosidase (α-fuc) as a biomarker for cellular senescence and the development of an α-fuc-responsive aggregation induced emission (AIE) probe, termedQM-NHαfucdesigned to complement more conventional probes based on β-galactosidase (β-gal). UsingQM-NHαfuc, the onset of replicative-, reactive oxygen species (ROS)-, ultraviolet A (UVA)-, and drug-induced senescence could be probed effectively.QM-NHαfucalso proved capable of identifying senescent cells lacking β-gal expression. The non-invasive real-time senescence tracking provided byQM-NHαfucwas validated in anin vivosenescence model. The results presented in this study lead us to suggest that theQM-NHαfuccould emerge as a useful tool for investigating senescence processes in biological systems.",

author = "Seyoung Koo and Miae Won and Hao Li and Kim, {Won Young} and Mingle Li and Chenxu Yan and Amit Sharma and Zhiqian Guo and Zhu, {Wei Hong} and Sessler, {Jonathan L.} and Lee, {Jin Yong} and Kim, {Jong Seung}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (CRI project no. 2018R1A3B1052702, 2019M3E5D1A01068998, J. S. K. and Brain Pool Program Grant No. 2020H1D3A1A02080172, M. L.), Basic Science Research Program (2020R1A6A3A01100551, M. W. and 2020R1A6A3A01100558, S. K., and 2019R1A6A1A10073079, J. Y. L). We are thankful for National Research Foundation of China (21788102) and a Department of Biotechnology, New Delhi (Ramalingaswami Fellowship 2019, Grant No. BT/RLF/Re-entry/59/2018, A. S.). Support from the Robert A. Welch Foundation (F-0018 to J. L. S.) is acknowledged. We also express appreciation to O. S. Shin (Korea University Guro Hospital) for a gift of the human dermal fibroblast (HDF) cell line. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = aug,

day = "7",

doi = "10.1039/d1sc02259h",

language = "English",

volume = "12",

pages = "10054--10062",

journal = "Chemical Science",

issn = "2041-6520",

publisher = "Royal Society of Chemistry",

number = "29",

}

TY - JOUR

T1 - Harnessing α-l-fucosidase forin vivocellular senescence imaging

AU - Koo, Seyoung

AU - Won, Miae

AU - Li, Hao

AU - Kim, Won Young

AU - Li, Mingle

AU - Yan, Chenxu

AU - Sharma, Amit

AU - Guo, Zhiqian

AU - Zhu, Wei Hong

AU - Sessler, Jonathan L.

AU - Lee, Jin Yong

AU - Kim, Jong Seung

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (CRI project no. 2018R1A3B1052702, 2019M3E5D1A01068998, J. S. K. and Brain Pool Program Grant No. 2020H1D3A1A02080172, M. L.), Basic Science Research Program (2020R1A6A3A01100551, M. W. and 2020R1A6A3A01100558, S. K., and 2019R1A6A1A10073079, J. Y. L). We are thankful for National Research Foundation of China (21788102) and a Department of Biotechnology, New Delhi (Ramalingaswami Fellowship 2019, Grant No. BT/RLF/Re-entry/59/2018, A. S.). Support from the Robert A. Welch Foundation (F-0018 to J. L. S.) is acknowledged. We also express appreciation to O. S. Shin (Korea University Guro Hospital) for a gift of the human dermal fibroblast (HDF) cell line. Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2021/8/7

Y1 - 2021/8/7

N2 - Precise detection of cellular senescence may allow its role in biological systems to be evaluated more effectively, while supporting studies of therapeutic candidates designed to evade its detrimental effect on physical function. We report here studies of α-l-fucosidase (α-fuc) as a biomarker for cellular senescence and the development of an α-fuc-responsive aggregation induced emission (AIE) probe, termedQM-NHαfucdesigned to complement more conventional probes based on β-galactosidase (β-gal). UsingQM-NHαfuc, the onset of replicative-, reactive oxygen species (ROS)-, ultraviolet A (UVA)-, and drug-induced senescence could be probed effectively.QM-NHαfucalso proved capable of identifying senescent cells lacking β-gal expression. The non-invasive real-time senescence tracking provided byQM-NHαfucwas validated in anin vivosenescence model. The results presented in this study lead us to suggest that theQM-NHαfuccould emerge as a useful tool for investigating senescence processes in biological systems.

AB - Precise detection of cellular senescence may allow its role in biological systems to be evaluated more effectively, while supporting studies of therapeutic candidates designed to evade its detrimental effect on physical function. We report here studies of α-l-fucosidase (α-fuc) as a biomarker for cellular senescence and the development of an α-fuc-responsive aggregation induced emission (AIE) probe, termedQM-NHαfucdesigned to complement more conventional probes based on β-galactosidase (β-gal). UsingQM-NHαfuc, the onset of replicative-, reactive oxygen species (ROS)-, ultraviolet A (UVA)-, and drug-induced senescence could be probed effectively.QM-NHαfucalso proved capable of identifying senescent cells lacking β-gal expression. The non-invasive real-time senescence tracking provided byQM-NHαfucwas validated in anin vivosenescence model. The results presented in this study lead us to suggest that theQM-NHαfuccould emerge as a useful tool for investigating senescence processes in biological systems.

UR - http://www.scopus.com/inward/record.url?scp=85111602632&partnerID=8YFLogxK

U2 - 10.1039/d1sc02259h

DO - 10.1039/d1sc02259h

M3 - Article

AN - SCOPUS:85111602632

SN - 2041-6520

VL - 12

SP - 10054

EP - 10062

JO - Chemical Science

JF - Chemical Science

IS - 29

ER -

Harnessing α-l-fucosidase forin vivocellular senescence imaging

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