HCV-induced PKR activation is stimulated by the mitogen- and stress-activated protein kinase MSK2

Ju Il Kang, Byung Yoon Ahn

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The replication of viral nucleic acids triggers cellular antiviral responses. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) plays a key role in this antiviral response. We have recently reported that JFH-1 HCV replication in Huh-7 cells triggers PKR activation. Here we show that the HCV-induced PKR activation is further stimulated by the mitogen- and stress-activated protein kinase 2 (MSK2), a member of the 90. kDa ribosomal S6 kinase (RSK) family that has emerged as an important downstream effector of ERK and p38 MAPK signaling pathways. We show that MSK2 binds PKR and stimulates PKR phosphorylation, whereas the closely related MSK1 and RSK2 have no effect. Our data further indicate that MSK2 functions as an adaptor in mediating PKR activation, apparently independent of its catalytic activity. These results suggest that, in addition to viral dsRNA, stress signaling contributes to the regulation of cellular antiviral response.

Original languageEnglish
Pages (from-to)248-253
Number of pages6
JournalBiochemical and biophysical research communications
Volume407
Issue number1
DOIs
Publication statusPublished - 2011 Apr 1

Keywords

  • DsRNA signaling
  • HCV
  • IFN
  • PKR
  • RSK

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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