Abstract
Heat-killed lactic acid bacteria (LAB) are known to be important immunomodulators that stimulate tumor necrosis factor-α (TNF-α) and nitric oxide (NO) production as well as increase phagocytic activity in macrophages. NK cells play a critical role in innate immune response and induce spontaneous killing of tumor cells and virus-infected cells. However, the effect of heat-killed LAB on NK cells is still unclear. In this study, we investigated the effect of heat-killed Lactobacillus acidophilus La205 (La205) on NK cytolytic activity. We found that heat-killed La205 directly stimulated NK cytolytic activity in dose- and time-dependent manners. To determine the mechanism underlying heat-killed La205-enhanced NK cytotoxicity, the expression of NK activating receptors was tested. Heat-killed La205 did not affect the expression of NK activating receptors. To investigate whether NK degranulation is related to heat-killed La205-enhanced NK cytotoxicity, NK degranulation inhibitor concanamycin A (CMA) was used. CMA effectively blocked heat-killed La205-induced NK cytotoxicity, and an assay for detection of a degranulation marker, CD107a, showed that heat-killed La205 increased granule exocytosis approximately 2-fold in comparison to non-treated NK cells. In addition, heat-killed La205 dramatically elevated mRNA expression of granulysin, a component of the cytolytic granule contents, in NK cells. However, other granule contents, including perforin and granzymes, were not changed by heat-killed La205. From these data, we concluded that heat-killed La205 stimulated NK cytolytic activity through enhancement of granule exocytosis, and granulysin may be a critical mediator in heat-killed La205-induced granule exocytosis.
| Original language | English |
|---|---|
| Pages (from-to) | 171-176 |
| Number of pages | 6 |
| Journal | Immunology Letters |
| Volume | 136 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2011 May 1 |
| Externally published | Yes |
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Keywords
- Cytotoxicity
- Granule exocytosis
- Granulysin
- Lactic acid bacteria (LAB)
- Lactobacillus acidophilus La205 (La205)
- NK cell
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Heat-killed Lactobacillus acidophilus La205 enhances NK cell cytotoxicity through increased granule exocytosis. / Cheon, Soyoung; Lee, Ki Woong; Kim, Kyung Eun; Park, Jung Kyu; Park, Sunyoung; Kim, Chul hyun; Kim, Daejin; Lee, Hyong Joo; Cho, Dae Ho.
In: Immunology Letters, Vol. 136, No. 2, 01.05.2011, p. 171-176.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heat-killed Lactobacillus acidophilus La205 enhances NK cell cytotoxicity through increased granule exocytosis
AU - Cheon, Soyoung
AU - Lee, Ki Woong
AU - Kim, Kyung Eun
AU - Park, Jung Kyu
AU - Park, Sunyoung
AU - Kim, Chul hyun
AU - Kim, Daejin
AU - Lee, Hyong Joo
AU - Cho, Dae Ho
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Heat-killed lactic acid bacteria (LAB) are known to be important immunomodulators that stimulate tumor necrosis factor-α (TNF-α) and nitric oxide (NO) production as well as increase phagocytic activity in macrophages. NK cells play a critical role in innate immune response and induce spontaneous killing of tumor cells and virus-infected cells. However, the effect of heat-killed LAB on NK cells is still unclear. In this study, we investigated the effect of heat-killed Lactobacillus acidophilus La205 (La205) on NK cytolytic activity. We found that heat-killed La205 directly stimulated NK cytolytic activity in dose- and time-dependent manners. To determine the mechanism underlying heat-killed La205-enhanced NK cytotoxicity, the expression of NK activating receptors was tested. Heat-killed La205 did not affect the expression of NK activating receptors. To investigate whether NK degranulation is related to heat-killed La205-enhanced NK cytotoxicity, NK degranulation inhibitor concanamycin A (CMA) was used. CMA effectively blocked heat-killed La205-induced NK cytotoxicity, and an assay for detection of a degranulation marker, CD107a, showed that heat-killed La205 increased granule exocytosis approximately 2-fold in comparison to non-treated NK cells. In addition, heat-killed La205 dramatically elevated mRNA expression of granulysin, a component of the cytolytic granule contents, in NK cells. However, other granule contents, including perforin and granzymes, were not changed by heat-killed La205. From these data, we concluded that heat-killed La205 stimulated NK cytolytic activity through enhancement of granule exocytosis, and granulysin may be a critical mediator in heat-killed La205-induced granule exocytosis.
AB - Heat-killed lactic acid bacteria (LAB) are known to be important immunomodulators that stimulate tumor necrosis factor-α (TNF-α) and nitric oxide (NO) production as well as increase phagocytic activity in macrophages. NK cells play a critical role in innate immune response and induce spontaneous killing of tumor cells and virus-infected cells. However, the effect of heat-killed LAB on NK cells is still unclear. In this study, we investigated the effect of heat-killed Lactobacillus acidophilus La205 (La205) on NK cytolytic activity. We found that heat-killed La205 directly stimulated NK cytolytic activity in dose- and time-dependent manners. To determine the mechanism underlying heat-killed La205-enhanced NK cytotoxicity, the expression of NK activating receptors was tested. Heat-killed La205 did not affect the expression of NK activating receptors. To investigate whether NK degranulation is related to heat-killed La205-enhanced NK cytotoxicity, NK degranulation inhibitor concanamycin A (CMA) was used. CMA effectively blocked heat-killed La205-induced NK cytotoxicity, and an assay for detection of a degranulation marker, CD107a, showed that heat-killed La205 increased granule exocytosis approximately 2-fold in comparison to non-treated NK cells. In addition, heat-killed La205 dramatically elevated mRNA expression of granulysin, a component of the cytolytic granule contents, in NK cells. However, other granule contents, including perforin and granzymes, were not changed by heat-killed La205. From these data, we concluded that heat-killed La205 stimulated NK cytolytic activity through enhancement of granule exocytosis, and granulysin may be a critical mediator in heat-killed La205-induced granule exocytosis.
KW - Cytotoxicity
KW - Granule exocytosis
KW - Granulysin
KW - Lactic acid bacteria (LAB)
KW - Lactobacillus acidophilus La205 (La205)
KW - NK cell
UR - http://www.scopus.com/inward/record.url?scp=79953028277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953028277&partnerID=8YFLogxK
U2 - 10.1016/j.imlet.2011.01.007
DO - 10.1016/j.imlet.2011.01.007
M3 - Article
C2 - 21256158
AN - SCOPUS:79953028277
VL - 136
SP - 171
EP - 176
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 2
ER -