Heat shock protein 27 interacts with vimentin and prevents insolubilization of vimentin subunits induced by cadmium

Jae Seon Lee, Mei Hua Zhang, Eun Kyung Yun, Dongho Geum, Kyungjin Kim, Tae Hyung Kim, Yun Sook Lim, Jeong Sun Seo

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Vimentin is an intermediate filament that regulates cell attachment and subcellular organization. In this study, vimentin filaments were morphologically altered, and its soluble subunits were rapidly reduced via cadmium chloride treatment. Cadmium chloride stimulated three major mitogen-activated protein kinases (MAPKs): extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, and led apoptotic pathway via caspase-9 and caspase-3 activations. In order to determine whether MAPKs were involved in this cadmium-induced soluble vimentin disappearance, we applied MAPK-specific inhibitors (PD98059, SP600125, SB203580). These inhibitors did not abolish the cadmium-induced soluble vimentin disappearance. Caspase and proteosome degradation pathway were also not involved in soluble vimentin disappearance. When we observed vimentin levels in soluble and insoluble fractions, soluble vimentin subunits shifted to an insoluble fraction. As we discovered that heat-shock protein 27 (HSP27) was colocalized and physically associated with vimentin in unstressed cells, the roles of HSP27 with regard to vimentin were assessed. HSP27-overexpressing cells prevented morphological alterations of the vimentin filaments, as well as reductions of soluble vimentin, in the cadmium-treated cells. Moreover, HSP27 antisense oligonucleotide augmented these cadmium-induced changes in vimentin. These findings indicate that HSP27 prevents disruption of the vimentin intermediate filament networks and soluble vimentin disappearance, by virtue of its physical interaction with vimentin in cadmium-treated SK-N-SH cells.

Original languageEnglish
Pages (from-to)427-435
Number of pages9
JournalExperimental and Molecular Medicine
Volume37
Issue number5
DOIs
Publication statusPublished - 2005 Oct 31

Keywords

  • Apoptosis
  • Cadmium chloride
  • Heat shock protein
  • Mitogen activated protein kinases
  • Molecular chaperones
  • Vimentin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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